Regulation of the appearance of cytoplasmic RNAs from region 1 of the adenovirus 2 genome.
Regulation of the appearance of cytoplasmic RNAs from region 1 of the adenovirus 2 genome.
复制标题
腺病毒 2 基因组第 1 区细胞质 RNA 出现的调节。
DOI：
10.1016/0022-2836(78)90048-7
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发表时间：
1978
影响因子：
5.6
通讯作者：
H. Raskas
中科院分区：
文献类型：
--
作者：
David J. Spector;Michael McGrogan;H. Raskas
用特异的DNA探针检测了由腺病毒2基因组9-10.7区(早期区1)编码的细胞质RNA物种出现的时间过程。与病毒DNA限制性内切酶片段的杂交可分解5个细胞质RNA。一个22的S RNA包含在MAP位置4.4右侧的序列。一个13 S RNA(13SL)映射到该位点的左侧，而一个类似大小的RNA(13SF)从4.4%的两个单独区域转录而来。每13个S RNA都有3‘端序列，其中有9个明显的S RNA。在22个S种中也存在13SfRNA的序列。在生产性感染期间，每一种核糖核酸在细胞质中都有其特有的出现率；22名S和13名单链核糖核酸在整个感染过程中以恒定的速度积累。最初，13SfRNA的出现速度大大低于22个S和13个SL，RNA。在后期，13个SfRNA的积累被刺激50倍以上，直到它的出现超过其他“早期”RNA。只有在病毒DNA复制开始后才能检测到这9个S RNA。它们的标记率接近其共线13 S RNA的标记率。结果表明，区域1的RNA存在复杂的调控模式。调控事件必须解释：(1)早期RNA的后期继续合成；(2)13SfRNA的增加；(3)9 S合成的开启；(4)晚期共线13 S和9 S RNA的平衡出现速率。
The time-course of appearance of cytoplasmic RNA species coded by the region 9 to 10.7 on the adenovirus 2 genome (early region 1) has been examined using specific DNA probes. Hybridization with restriction endonuclease fragments of viral DNA resolved five cytoplasmic RNAs. A 22 S RNA contains sequences to the right of map position 4.4. One 13 S RNA (13 SL) maps to the left of this site, while an RNA of similar size (13 Sf) is transcribed from two separate regions to the right of 4.4. Each 13 S RNA shares 3′ sequences with a distinct 9 S RNA. Sequences in 13SfRNA are also present in the 22 S species. These mapping studies have allowed the identification of probes that can be used to assay each RNA after fractionation by size.During productive infection, each RNA species has a characteristic rate of appearance in the cytoplasm; 22 S and 13 SLRNA accumulate at a constant rate throughout infection. Initially 13 SfRNA appears at a rate substantially lower than the 22 S and 13 SL, RNAs. At late times accumulation of 13 SfRNA is stimulated more than 50-fold, until its appearance exceeds that of the other “early” RNAs. The 9 S RNAs are detected only after viral DNA replication begins. The rate of labeling of each approaches that of its colinear 13 S RNA. The results demonstrate a complex regulatory pattern for region 1 RNAs. Regulatory events must account for: (1) continued synthesis of early RNAs at late times; (2) increased accumulation of 13 SfRNA; (3) the turn on of 9 S synthesis; and (4) balanced rates of appearance of the colinear 13 S and 9 S RNAs at late times.