MAINTENANCE AND REVERSIBILITY OF ACTIVE ALBUMIN SECRETION BY ADULT-RAT HEPATOCYTES CO-CULTURED WITH ANOTHER LIVER EPITHELIAL-CELL TYPE
MAINTENANCE AND REVERSIBILITY OF ACTIVE ALBUMIN SECRETION BY ADULT-RAT HEPATOCYTES CO-CULTURED WITH ANOTHER LIVER EPITHELIAL-CELL TYPE
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DOI：
10.1016/0014-4827(83)90107-6
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发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
GUILLOUZO, A
中科院分区：
文献类型：
--
作者：
GUGUENGUILLOUZO, C;CLEMENT, B;GUILLOUZO, A
将成年大鼠肝细胞与另一种肝上皮细胞在含或不含胎牛血清的培养液中共培养，发现：肝细胞存活时间> 2个月;在整个培养期间，白蛋白分泌水平保持高水平;降低的分泌可能被逆转;并且该蛋白分泌活性似乎依赖于细胞-细胞接触的存在和细胞外物质的产生。结果首次证明了在无血清培养基中培养的成年肝细胞的高水平特异性功能（白蛋白分泌）的长期稳定性和可逆性，并表明其他肝细胞类型的存在和细胞外基质的产生都是维持培养肝细胞特异性功能所必需的。
When adult rat hepatocytes were cocultured with another liver epithelial cell type in a medium supplemented or not with fetal calf serum (FCS), it was found that: they survived for > 2 mo.; albumin secretion levels remained high over the whole culture period; decreased secretion might be reversed; and this protein secretion activity appeared to be dependent upon both the presence of cell-cell contacts and the production of an extracellular material. The results demonstrate for the 1st time long-term stabilization and reversibility of a specific function (albumin secretion) at high levels by adult hepatocytes cultured in serum-free medium, and suggest that both the presence of other liver cell type(s) and the production of an extracellular matrix are needed for the maintenance of specific functions in cultured hepatocytes.