Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis
Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis
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拟南芥中转录因子 AtMYB103 通过调节绒毡层发育、胼胝质溶解和外壁形成来促进花药发育
DOI：
10.1111/j.1365-313x.2007.03254.x
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发表时间：
2007-11-01
期刊：
影响因子：
7.2
通讯作者：
Yang, Zhong-Nan
中科院分区：
文献类型：
--
作者：
Zhang, Zai-Bao;Zhu, Jun;Yang, Zhong-Nan
利用转基因技术下调转录因子AtMYB103可导致拟南芥花药发育过程中绒毡层早期退化和花粉畸变。本文描述了AtMYB103基因在三个敲除突变体中的功能分析。采用甲基磺酸乙酯（EMS）诱变制备了两个雄性不育突变体ms188-1和ms188-2。利用图谱克隆方法，将ms188定位到5号染色体上含有AtMYB103转录因子的95.8 kb区域。序列分析显示，ms188-1在AtMYB103编码区有一个提前成熟的终止密码子，而ms188-2在AtMYB103的第一个外显子上有一个CCT -> CTT碱基对变化，导致R2R3结构域的脯氨酸被亮氨酸残基取代。第三个突变体AtMYB103转座子标记系也表现出雄性不育表型。等位基因检测表明，MS188和AtMYB103属于同一基因座。细胞学观察显示ms188植物绒毡层发育缺陷和胼胝质溶解改变。成熟花药中大部分小孢子降解，存活的小孢子缺乏外壁。AtMYB103编码的R2R3 MYB蛋白主要位于细胞核中。Real-time RT-PCR分析表明，callase相关基因A6受AtMYB103调控。突变花药中未检测到外叶形成基因MS2的表达。这些结果提示AtMYB103在拟蓝花药绒毡层发育、胼胝质溶解和外壁形成中起重要作用。
Downregulation of the transcription factor AtMYB103 using transgenic technology results in early tapetal degeneration and pollen aberration during anther development in Arabidopsis thaliana. This paper describes the functional analysis of the AtMYB103 gene in three knock-out mutants. Two male sterile mutants, ms188-1 and ms188-2, were generated by ethyl-methane sulfonate (EMS) mutagenesis. A map-based cloning approach was used, and ms188 was mapped to a 95.8-kb region on chromosome 5 containing an AtMYB103 transcription factor. Sequence analysis revealed that ms188-1 had a pre-mature stop codon in the AtMYB103 coding region, whereas ms188-2 had a CCT -> CTT base-pair change in the first exon of AtMYB103, which resulted in the replacement of a proline by a leucine residue in the R2R3 domain. The third mutant, an AtMYB103 transposon-tagging line, also showed a male sterile phenotype. Allelism tests indicated that MS188 and AtMYB103 belong to the same locus. Cytological observation revealed defective tapetum development and altered callose dissolution in ms188 plants. Additionally, most of the microspores in mature anthers were degraded and surviving microspores lacked exine. AtMYB103 encoded an R2R3 MYB protein that is predominantly located in the nucleus. Real-time RT-PCR analysis indicated that the callase-related gene A6 was regulated by AtMYB103. Expression of the exine formation gene MS2 was not detected in mutant anthers. These results implicate that AtMYB103 plays an important role in tapetum development, callose dissolution and exine formation in A. thaliana anthers.