Sensitization of human glioblastoma T98G cells to VP16 and VM26 by human tumor necrosis factor.
Sensitization of human glioblastoma T98G cells to VP16 and VM26 by human tumor necrosis factor.
复制标题
人肿瘤坏死因子对人胶质母细胞瘤 T98G 细胞对 VP16 和 VM26 的敏感性。
作者：
P. Morgavi;G. Cimoli;C. Ottoboni;A. Michelotti;P. Conte;S. Parodi;P. Russo
以表达肿瘤坏死因子受体的人胶质母细胞瘤细胞系T98G为研究对象，研究了肿瘤坏死因子对VP16或VM26细胞毒作用的影响。T98G细胞虽然不产生内源性的肿瘤坏死因子，但对肿瘤坏死因子的杀伤作用具有抵抗力。T98G细胞对VP16或VM26的作用也呈中度敏感。浓度为1000U/ml的肿瘤坏死因子可显著提高细胞对药物的敏感性。这种作用是由于肿瘤坏死因子使VP16诱导的可切割复合体增加所致。这些结果表明，肿瘤坏死因子特异性地增敏人脑胶质瘤T98G细胞对VM26的VP16的作用。
The effect of Tumor Necrosis Factor (TNF) on VP16 or VM26 cytotoxicity was studied in a human glioblastoma cell line T98G, which expresses TNF-receptors. Although T98G cells did not produce TNF endogenously they were resistant to the cytolytic effect of TNF. T98G cells were also moderately sensitive to the action of VP16 or VM26. TNF given at 1000 U/ml was able to increase the cell drug-sensitivity significantly. This effect was due to an increase in the VP 16-induced cleavable-complexes by TNF. These findings suggest that TNF specifically sensitizes human glioma T98G cells to the effects of VP 16 of VM26.