Elimination of neurokinin-1 receptor neurons in caudal nucleus reverses the effects of systemic bicuculline on c-Fos expression in rat trigeminal sensory nucleus: I. High intensity electrical stimulation of the trigeminal ganglion
Elimination of neurokinin-1 receptor neurons in caudal nucleus reverses the effects of systemic bicuculline on c-Fos expression in rat trigeminal sensory nucleus: I. High intensity electrical stimulation of the trigeminal ganglion
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DOI：
10.1016/j.neuroscience.2005.03.021
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发表时间：
2005-12
期刊：
影响因子：
3.3
通讯作者：
T. Abe;N. Ohshita;S. Sugiyo;M. Moritani;M. Kobayashi;M. Takemura
中科院分区：
文献类型：
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作者：
T. Abe;N. Ohshita;S. Sugiyo;M. Moritani;M. Kobayashi;M. Takemura
虽然神经激肽-1受体（NK-1）的神经元分布在三叉神经尾侧核（Vc）的第一层，并构成主要的投射神经元，很少有人知道他们的基本作用（S）在伤害性加工。本研究探讨了枕大池内注射P物质（SP）结合皂草素的作用（SP-Sap; 5μM，5μl）[联合或不联合荷包牡丹碱全身给药]对电刺激三叉神经节（TG）10 min后2 h三叉神经感觉核（TSN）c-Fos表达的影响。SP-Sap处理组大鼠Vc第I、III层NK-1免疫阳性神经元数量明显少于生理盐水（5μl）或空白皂草素（5μM，5μl）处理组。在Sal或Bl-Sap处理的控制，高强度刺激诱导c-Fos表达的神经元在整个范围内的同侧浅层的Vc（Vc I/II），大细胞区的Vc（Vc III/IV）和背侧或背内侧分区的吻侧TSN以上的obex（三叉神经的主要，口头（Vo）和极间核）。荷包牡丹碱（2 mg/kg，i. p.）在Sal或Bl-Sap处理的对照中，VcI/II、VcIII/IV和Vo中的c-Fos免疫阳性神经元的数量减少。与此相反，高强度的刺激诱导较少的c-Fos免疫阳性神经元的Vc I/II和Vo的大鼠与SP-Sap处理与Sal或BL-Sap处理的对照组相比。在SP-Sap处理的大鼠预先给予荷包牡丹碱，c-Fos免疫阳性神经元的数目增加，在VcI/II和Vo与SP-Sap处理的大鼠预先给予Sal。这些结果表明，NK-1免疫阳性神经元在第一和第三层的Vc发挥关键作用的伤害性特异性处理的TSN通过GABAA受体。
Although neurokinin-1 receptor (NK-1)-bearing neurons are distributed in lamina I of the trigeminal caudal nucleus (Vc) and constitute major projection neurons, little is known about their fundamental role(s) in nociceptive processing. This study examines the effect of intra cisterna magna injection of substance P (SP) conjugated to saporin (SP-Sap; 5μM, 5μl) [with/without systemic administration of bicuculline] on c-Fos expression in the trigeminal sensory nucleus (TSN) induced 2 h after 10 min repetitive electrical stimulation of the trigeminal ganglion (TG) at high intensity (1.0mA, 5Hz, 5 ms) in the urethane-anesthetized rat. In the SP-Sap-treated rats, the numbers of NK-1-immunopositive neurons in laminae I and III of the Vc decreased compared with rats similarly pretreated with saline (Sal; 5μl) or blank-saporin (Bl-Sap; 5μM, 5μl). In Sal- or Bl-Sap-treated controls, high intensity stimulation induced c-Fos expression in neurons throughout the full extent of ipsilateral superficial layers of the Vc (VcI/II), magnocellular zone of the Vc (VcIII/IV) and the dorsal or dorsomedial subdivisions of the rostral TSN above the obex (trigeminal principal, oral (Vo) and interpolar nuclei). Preadministration of bicuculline (2mg/kg, i.p.) decreased the numbers of c-Fos-immunopositive neurons in the VcI/II, VcIII/IV and Vo in Sal- or Bl-Sap-treated controls. In contrast, high intensity stimulation induced less c-Fos-immunopositive neurons in the VcI/II and Vo of rats treated with SP-Sap compared with those in Sal- or Bl-Sap-treated controls. In SP-Sap-treated rats preadministered with bicuculline, the numbers of c-Fos-immunopositive neurons in the VcI/II and Vo were increased compared with the SP-Sap-treated rats preadministered with Sal. These results suggest that NK-1-immunopositive neurons in laminae I and III of Vc play a pivotal role in the nociceptive specific processing in the TSN through GABAAreceptors.