In vitro antioxidant properties of dantrolene sodium
In vitro antioxidant properties of dantrolene sodium
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DOI：
10.1006/phrs.2001.0890
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发表时间：
2001-12-01
影响因子：
9.3
通讯作者：
Küfrevioglu, Ö
中科院分区：
文献类型：
--
作者：
Büyükokuroglu, ME;Gülçin, I;Küfrevioglu, Ö
丹曲林钠是一种骨骼肌松弛剂，可抑制肌浆网的细胞内Ca 2+释放。本研究的目的是检查丹曲林钠可能的体外抗氧化作用。为此，采用硫氰酸盐法研究了丹曲林钠的体外抗氧化作用。此外，还测定了还原力和自由基清除活性。丹曲林钠在亚油酸乳剂体系中表现出较强的抗氧化活性。抗氧化活性随着丹曲林钠（50、100、250 μ g）的量的增加而增加。丹曲林钠的50、100和250 μ g样品分别对亚油酸的过氧化反应显示出55%、70%和82%的抑制作用。另一方面，250 μ g α-生育酚样品显示出62%的亚油酸过氧化抑制。与抗氧化活性一样，丹曲林钠的还原能力也以剂量依赖性方式增加。丹曲林的还原能力与对照组相比具有统计学显著性，但低于丁基羟基甲苯（BHT）和槲皮素。虽然丹曲林钠具有自由基清除活性，但无统计学显著性。与丹曲林钠相比，槲皮素和丁基羟基茴香醚（BHA）具有高度有效的自由基清除活性，并且具有统计学显著性。根据这些结果，可以说丹曲林钠的抗氧化作用与其在亚油酸乳剂中的抗氧化活性和还原力有关，而不是其清除自由基的活性。这些性质可能是抑制脂质过氧化的主要原因。(C)北京：科学出版社.
Dantrolene sodium is a skeletal muscle relaxant, which inhibits intracellular Ca2+ release from the sarcoplasmic reticulum. The aim of this study is to examine possible in vitro antioxidant effects of dantrolene sodium. For this reason, the in vitro antioxidant effects of dantrolene sodium were studied using thiocyanate methods. Additionally, the reducing power and free radical scavenging activity were determined. Dantrolene sodium showed strong antioxidant activity in the linoleic acid emulsion system. The antioxidant activity increased with an increasing amount of dantrolene sodium (50, 100, 250 mug). The 50, 100 and 250 mug samples of dantrolene sodium showed 55%, 70% and 82% inhibition on peroxidation of linoleic acid, respectively. On the other hand, the 250 mug sample of alpha -tocopherol showed 62% inhibition of peroxidation of linoleic acid. Like antioxidant activity, the reducing power of dantrolene sodium increased in a dose-dependent manner. The reducing power of dantrolene was statistically significant vs control, but lower than butylated hydroxytoluene (BHT) and quercetin. Although dantrolene sodium had free radical scavenging activity this was not statistically significant. In contrast to dantrolene sodium, quercetin and butylated hydroxyanisole (BHA) had highly potent free radical scavenging activities and those were statistically significant. According to the these results, it may be said that antioxidant effect of dantrolene sodium is more related to its antioxidant activity in linoleic acid emulsion and reducing power, than to its free radical scavenging activity. These properties may be major reasons for the inhibition of lipid peroxidation. (C) 2001 Academic Press.