Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells
Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells
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DOI：
10.1016/s0142-9612(00)00220-9
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发表时间：
2001-07-01
期刊：
影响因子：
14
通讯作者：
Rao, KP
中科院分区：
文献类型：
--
作者：
Shanmugasundaram, N;Ravichandran, P;Rao, KP
以胶原蛋白和壳聚糖为原料，以互穿聚合物网络（IPN）的形式制备了一种可生物降解的聚合物支架，用于人表皮样癌细胞（HEp-2，辛辛那提）的体外培养。戊二醛作为交联剂用于支架的开发。使用胶原蛋白和壳聚糖溶液以3：7、4：6、5：5、6：4和7：3（胶原蛋白：壳聚糖）的比例的不同比例的混合物制备各种类型的支架。采用傅里叶变换红外光谱（FT-IR）、差示扫描量热法（DSC）和热重分析（TGA）对支架进行了表征。在生理pH（7.4）的磷酸盐缓冲液中进行平衡溶胀研究，以研究其在弱碱性pH下的溶胀特性。选择表现出最佳溶胀性能的支架作为进行体外培养研究的最佳支架。使用HEp-2细胞在选定的支架上进行体外培养研究，并通过在不同培养天数以不同放大倍数拍摄的光学照片确定其生长形态。上述研究结果表明，由胶原和壳聚糖制备的支架可以用作培养HEp-2细胞的基质，也可以用作体外模型来测试抗癌药物。(C)2001爱思唯尔科技有限公司版权所有。
A biodegradable polymer scaffold was developed using collagen and chitosan, in the form of interpenetrating polymeric network (IPN), for in vitro culture of human epidermoid carcinoma cells (HEp-2, Cincinnati). Glutaraldehyde was used as cross-linking agent For the development of scaffold. Various types of scaffolds were prepared using different proportionate mixtures of collagen and chitosan solutions in the ratio of 3:7, 4:6, 5:5, 6:4 and 7:3 (collagen:chitosan). These scaffolds were fully characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA). Equilibrium swelling studies were carried out in phosphate buffer of physiological pH (7.4) to study its swelling characteristics at slightly alkaline pH. The scaffold that showed optimum swelling property was selected as the best scaffold for performing in vitro culture studies. In vitro culture studies were carried out using HEp-2 cells, over the selected scaffold and its growth morphology was determined through optical photographs taken at different magnifications at various days of culture. The results of the above studies suggest that the scaffolds prepared from collagen and chitosan can be utilized as a substrate to culture HEp-2 cells and can also be used as an in vitro model to test anticancerous drugs. (C) 2001 Elsevier Science Ltd. All rights reserved.