Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides.
Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides.
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DOI：
10.3390/ma9120994
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发表时间：
2016-12-08
期刊：
影响因子：
--
通讯作者：
Chung SJ
中科院分区：
文献类型：
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作者：
Choe W;Durgannavar TA;Chung SJ
抗体应用的迅速增加激发了多种新方法的开发，使用模拟 Fc 受体与抗体结合的智能生物材料来分离和靶向抗体。抗体的 Fc 结合结构域是效应蛋白和二抗等的主要结合位点，而抗原则与 Fab 区域结合。众所周知，蛋白 A、G 和 L 是病原菌表达的表面蛋白，可结合免疫球蛋白，并已广泛应用于抗体纯化策略中。当细菌蛋白用于抗体研究和应用时遇到一些困难。阻碍细菌蛋白使用的主要障碍之一是样品被微量这些蛋白污染，这会引起宿主的免疫反应。许多研究小组积极开发能够选择性且牢固地与抗体结合的合成配体。在已报道的配体中，与抗体 Fc 结构域结合的肽是抗体研究中颇具吸引力的工具。除了在抗体纯化色谱中用作高亲和力配体外，Fc 结合肽还可用于将抗体定位在纳米材料上并延长血清中蛋白质的半衰期。在这篇综述中，介绍了 Fc 结合肽的最新进展，并讨论了它们的结合特性和不同的应用。
The rapidly increasing application of antibodies has inspired the development of several novel methods to isolate and target antibodies using smart biomaterials that mimic the binding of Fc-receptors to antibodies. The Fc-binding domain of antibodies is the primary binding site for e.g., effector proteins and secondary antibodies, whereas antigens bind to the Fab region. Protein A, G, and L, surface proteins expressed by pathogenic bacteria, are well known to bind immunoglobulin and have been widely exploited in antibody purification strategies. Several difficulties are encountered when bacterial proteins are used in antibody research and application. One of the major obstacles hampering the use of bacterial proteins is sample contamination with trace amounts of these proteins, which can invoke an immune response in the host. Many research groups actively develop synthetic ligands that are able to selectively and strongly bind to antibodies. Among the reported ligands, peptides that bind to the Fc-domain of antibodies are attractive tools in antibody research. Besides their use as high affinity ligands in antibody purification chromatography, Fc-binding peptides are applied e.g., to localize antibodies on nanomaterials and to increase the half-life of proteins in serum. In this review, recent developments of Fc-binding peptides are presented and their binding characteristics and diverse applications are discussed.