BETA-ENDORPHIN IMMUNOREACTIVITY IN RAT AND HUMAN-BLOOD - RADIOIMMUNOASSAY, COMPARATIVE LEVELS AND PHYSIOLOGICAL ALTERATIONS
BETA-ENDORPHIN IMMUNOREACTIVITY IN RAT AND HUMAN-BLOOD - RADIOIMMUNOASSAY, COMPARATIVE LEVELS AND PHYSIOLOGICAL ALTERATIONS
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DOI：
10.1016/0024-3205(79)90250-9
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发表时间：
1979-01-01
期刊：
影响因子：
6.1
通讯作者：
LI, CH
中科院分区：
文献类型：
--
作者：
AKIL, H;WATSON, SJ;LI, CH
抗人β-内啡肽（β hEP）得自兔。1/1500稀释的结合[125I] β h-EP的抗血清显示有效范围为10pM-10nM。测定的灵敏度为2 - 3毫微微摩尔。该抗体与人β-内酰胺酶显示10 - 15%的交叉反应性。促脂素（β hLPH）。β-与垂体切除大鼠的血液相比，在未提取的样品中检测到大鼠血液中的EP样免疫反应性。在垂体切除动物的血浆中进行整个测定和校准曲线。β-在正常大鼠血浆中检测到EP样免疫反应性（75. ±. 15 fmole/ml），并且在肾上腺切除术后表现出显著增加（287. ±. 32 fmol/ml）。相比之下，来自5名健康正常人类男性的样品给出了接近测定的检测极限的值（12 fmol. ±. 3.9/ml血浆）。这样的值可能是由于抗血清与β h-LPH或其它循环激素的交叉反应性。相比之下，ACTH产生升高的患者和正常怀孕的人表现出显著升高的β-ACTH水平。血浆中的EP免疫反应性。
Antiserum against human .beta.-endorphin (.beta.hEP) was obtained from rabbit. The antiserum, diluted 1/1500 bound [125I] .beta.h-EP, demonstrated an effective range from 10pM-10nM. The sensitivity of the assay was 2-3 f[femto]moles. This antibody exhibited 10-15% cross-reactivity with human .beta.-lipotropin (.beta.hLPH). .beta.-EP-like immunoreactivity in rat blood was detected in unextracted samples when compared to blood from hypophysectomized rats. The whole assay and calibration curves were carried out in plasma from hypophysectomized animals. .beta.-EP-like immunoreactivity was detected in normal rat plasma (75 .+-. 15 fmole/ml), and exhibited substantial increases with adrenalectomy (287 .+-. 32 fmoles/ml). In contrast, samples from 5 healthy normal human males gave values near the limits of detection of the assay (12 fmoles .+-. 3.9/ml of plasma). Such values may be due to cross-reactivity of the antiserum with .beta.h-LPH or other circulating hormones. In contrast, patients with elevated ACTH production and normal pregnant humans exhibited significantly elevated levels of .beta.-EP immunoreactivity in plasma.