Performance and microbial communities of a continuous stirred tank anaerobic reactor treating two-phases olive mill solid wastes at low organic loading rates.
Performance and microbial communities of a continuous stirred tank anaerobic reactor treating two-phases olive mill solid wastes at low organic loading rates.
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DOI：
10.1016/j.jbiotec.2005.08.013
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发表时间：
2006-02
影响因子：
4.1
通讯作者：
B. Rincón;F. Raposo;R. Borja;J. M. González;M. C. Portillo;C. Saiz-Jimenez
中科院分区：
文献类型：
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作者：
B. Rincón;F. Raposo;R. Borja;J. M. González;M. C. Portillo;C. Saiz-Jimenez
在实验室规模上研究了连续搅拌槽式反应器（CSTR）处理两相橄榄油固体废弃物（OMSW）的性能和微生物群落结构。反应器在中温（35°C）和进水基质浓度为162 g总化学需氧量（COD）L-1和126 g挥发性固体（VS）L-1的条件下运行。在本工作中分析的数据对应于0.75和3.00 g CODL-1d-1之间的有机负荷率（OLR）的范围，得到去除效率在97.0- 95.6%的范围内。在试验范围内，当OLR增加时，甲烷产率从0.164 L CH 4Lreactor −1d− 1增加到0.659 L CH 4Lreactor −1d− 1。甲烷产率系数为0.225L CH 4g −1COD去除和0.290L CH 4g −1VS去除，几乎与OLR无关。还完成了该过程中所涉及的微生物群落的分子表征。通过16 S核糖体RNA基因的PCR扩增、变性梯度凝胶电泳（DGGE）、克隆和测序进行微生物种的分子鉴定。在生物反应器中的优势微生物中，厚壁菌门（以梭菌目为主要代表）是最丰富的类群，其次是绿弯菌和γ-变形菌门（以假单胞菌属为主要代表）。在生物反应器中检测到的其他细菌群是放线菌门、拟杆菌门和脱铁杆菌门。在古菌中，产甲烷菌Methanosaeta concilii是最具代表性的物种。
A study of the performance and microbial communities of a continuous stirred tank reactor (CSTR) treating two-phases olive mill solid wastes (OMSW) was carried out at laboratory-scale. The reactor operated at a mesophilic temperature (35°C) and an influent substrate concentration of 162g total chemical oxygen demand (COD)L−1and 126g volatile solids (VS)L−1. The data analyzed in this work corresponded to a range of organic loading rates (OLR) of between 0.75 and 3.00g CODL−1d−1, getting removal efficiencies in the range of 97.0–95.6%. Methane production rate increased from 0.164 to 0.659L CH4Lreactor−1d−1when the OLR increased within the tested range. Methane yield coefficients were 0.225L CH4g−1COD removed and 0.290L CH4g−1VS removed and were virtually independent of the OLR applied. A molecular characterization of the microbial communities involved in the process was also accomplished. Molecular identification of microbial species was performed by PCR amplification of 16S ribosomal RNA genes, denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. Among the predominant microorganisms in the bioreactor, the Firmicutes (mainly represented by Clostridiales) were the most abundant group, followed by the Chloroflexi and the Gamma-Proteobacteria (Pseudomonas species as the major representative). Other bacterial groups detected in the bioreactor were the Actinobacteria, Bacteroidetes and Deferribacteres. Among the Archaea, the methanogen Methanosaeta concilii was the most representative species.