Control of apatite crystal growth by the co-operative effect of a recombinant porcine amelogenin and fluoride
Control of apatite crystal growth by the co-operative effect of a recombinant porcine amelogenin and fluoride
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DOI：
10.1111/j.1600-0722.2006.00324.x
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发表时间：
2006-05-01
影响因子：
1.9
通讯作者：
Moradian-Oldak, Janet
中科院分区：
文献类型：
--
作者：
Iijima, Mayumi;Du, Chang;Moradian-Oldak, Janet
最近，我们使用从发育中的猪牙釉质中提取的天然釉原蛋白来研究氟和釉原蛋白对磷酸八钙(OCP)和磷灰石晶体生长的联合影响。本研究的目的是使用高纯度的重组釉原蛋白来研究这种联合作用。我们应用猪釉原蛋白(RP172)和氟化物作为牙釉质形成的模型。在该体系中，rP172与氟化物结合形成棒状磷灰石晶体。另一方面，在没有氟化物的情况下，可以形成尺寸相当的棒状OCP晶体，并且在没有釉原蛋白的情况下，OCP和磷灰石的混合晶体生长出了较大的六方棱柱。因此，高纯度和均一的重组釉原蛋白在F的协同作用下，以与提取的异质猪釉原蛋白相同的方式调节晶体的矿物相、习性和大小。我们认为，在这两种情况下，对晶相和形态的控制是釉原蛋白作为磷灰石矿化支架的直接影响。
Recently, we used native amelogenins extracted from developing pig enamel to examine the combined effect of fluoride and amelogenins on the growth of octacalcium phosphate (OCP) and apatite crystals. The purpose of the present study was to investigate this combined effect using a highly purified recombinant amelogenin. We applied porcine amelogenin (rP172) and fluoride in a dual-membrane system as a model for tooth enamel formation. The combination of rP172 and fluoride in this system resulted in the formation of rod-like apatite crystals. On the other hand, without fluoride, rod-like OCP crystals of a comparable size were formed, and rather large hexagonal prisms of mixed crystals of OCP and apatite grew without amelogenins. Thus, highly purified and homogeneous recombinant amelogenin, in co-operation with F, regulated the mineral phase, habit, and size of crystals in the same manner as the extracted heterogeneous porcine amelogenins. We suggest that in both cases the control over the crystal phase and morphology was a direct effect of amelogenin protein serving as a scaffold for apatite mineralization.