NEUTROPHILS SHOW CHEMOTAXIS TO TYPE-IV COLLAGEN AND ITS 7S DOMAIN AND CONTAIN A 67-KD TYPE-IV COLLAGEN BINDING-PROTEIN WITH LECTIN PROPERTIES
NEUTROPHILS SHOW CHEMOTAXIS TO TYPE-IV COLLAGEN AND ITS 7S DOMAIN AND CONTAIN A 67-KD TYPE-IV COLLAGEN BINDING-PROTEIN WITH LECTIN PROPERTIES
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DOI：
10.1165/ajrcmb/1.6.479
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发表时间：
1989-12-01
影响因子：
6.4
通讯作者：
MECHAM, RP
中科院分区：
文献类型：
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作者：
SENIOR, RM;HINEK, A;MECHAM, RP
试剂Ficoll-Hypaque、FMLP、HEPES、/3-乳糖、辛基-13-葡萄糖苷、DMSO、苯甲酰胺、e-氨基己酸(EACA)、PMSF和EDTA来自密苏里州圣路易斯Sigma化学公司。Affi-Gel 10来自Bio-Rad(加利福尼亚州里士满)。CM-纤维素(CM 52)来自Whatman(英国肯特郡)，DEAE-纤维素(Zeta Prep DE，60 mm圆盘)来自Cuno Inc.(美国康涅狄格州梅里登)。Trasylol®是拜耳股份公司(西德伍珀塔尔)赠送的礼物。细菌胶原酶(CLSPA)从Worthington(新泽西州弗里霍尔德)获得。层粘连蛋白是从EHS肿瘤中提纯的(12例)。为了产生弹性蛋白肽，将来自弹性蛋白产品(太平洋，密苏里州)的牛韧带弹性蛋白和人中性粒细胞弹性蛋白酶在37℃下以100：1(wt/wt)孵育过夜，并如前所述通过Trasylol-Sepharose层析法去除弹性蛋白酶(13)。如前所述获得了弹性蛋白受体(BCZ67)的单抗(14)。抗小鼠免疫球蛋白的过氧化物酶标记的山羊抗体来自印第安纳波利斯的Boehringer Mannheim。兔抗人IV型胶原多克隆抗体和7S胶原短链抗体的制备
Materials and MethodsReagents Ficoll-Hypaque, FMLP, HEPES,/3-lactose, octyl-13-glucoside, DMSO, benzamidine, e-aminocaproic acid (EACA), PMSF, and EDTA were from Sigma Chemical Co.(St. Louis, MO). Affi-Gel 10 was from Bio-Rad (Richmond, CA). CM-cellulose (CM 52) was from Whatman (Kent, UK) and DEAE-cellulose (ZETA Prep DE, 60-mm disc) was from CUNO Inc.(Meriden, CT). Trasylol® was a gift from Bayer AG (Wuppertal, West Germany). Bacterial collagenase (CLSPA) was obtained from Worthington (Freehold, NJ). Laminin was purified from EHS tumor (12). To generate elastin peptides, bovine ligament elastin and human neutrophil elastase, both from Elastin Products (Pacific, MO), were incubated at 100: 1 (wt/wt) overnight at 37 C and the elastase was removed by Trasylol-Sepharose chromatography as previously described (13). Monoclonal antibody to the elastin receptor (BCZ67) was obtained as previously described (14). Peroxidase-conjugated goat antibody against mouse immunoglobulin was from Boehringer Mannheim (Indianapolis, IN). Polyclonal antibodies to human type IV collagen and the short form of 7S collagen were raised in rabbits by standard