Reduction of doxyl stearates by ascorbate in unilamellar liposomes.
Reduction of doxyl stearates by ascorbate in unilamellar liposomes.
复制标题
单层脂质体中抗坏血酸还原硬脂酸多西酯。
DOI：
10.1016/0003-9861(90)90106-9
复制
发表时间：
1990
影响因子：
3.9
通讯作者：
Swartz,HM
中科院分区：
文献类型：
--
作者：
Sentjurc,M;Bacic,G;Swartz,HM
在大的单层脂质体中，研究了Doxyl基团在膜中的位置对外部抗坏血酸盐还原速率的影响。减少的速率增加的关键因素是氮氧化合物到水相的分区的程度; doxyl基团的接近膜的表面不是一个主要因素。与后者的发现一致，增加抗坏血酸的膜渗透速率的因素对所观察到的减少速率没有重大影响。我们的结论是，在这个系统中的外部水介质是减少的doxyl硬脂酸酯和doxyl硬脂酸酯之间的膜和水相的有效平衡的主要网站。
The effects of the position in the membrane of the doxyl group on the rate of reduction by external ascorbate were studied in large unilammelar liposomes. The key factor increasing the rate of reduction was the degree of partitioning of the nitroxide into the aqueous phase; the doxyl group's proximity to the surface of the membrane was not a major factor. Consistent with the latter finding, factors that increased the rate of membrane permeation by ascorbate did not have major effects on the observed rates of reduction. We conclude that in this system the external aqueous medium is the primary site of reduction of the doxyl stearates and the doxyl stearates are in effective equilibrium between the membrane and aqueous phases.