Phylogenetic diversity among geographically dispersed Chlamydiales endosymbionts recovered from clinical and environmental isolates of Acanthamoeba spp.
Phylogenetic diversity among geographically dispersed Chlamydiales endosymbionts recovered from clinical and environmental isolates of Acanthamoeba spp.
复制标题
DOI：
10.1128/aem.66.6.2613-2619.2000
复制
发表时间：
2000-06-01
影响因子：
4.4
通讯作者：
Gautom, RK
中科院分区：
文献类型：
--
作者：
Fritsche, TR;Horn, M;Gautom, RK
最近提出的衣原体目重组和新分类单元的描述正在扩大我们对这个曾经狭隘定义的分类单元的认识。我们在棘阿米巴属内共生了 4 株革兰氏阴性球菌，占棘阿米巴属的 5%。检查的分离株显示出衣原体成员典型的发育生命周期，其中一种内共生菌株被发现稳定感染从北美阿米巴角膜炎病例中回收的阿米巴分离株，另外三种在从北美（两个分离株）和欧洲（一个分离株）的环境来源中回收的棘阿米巴中发现。通过邻接法、简约法和距离矩阵法对这些分离株的近全长 16S rRNA 基因序列进行分析，揭示了它们与衣原体目的其他成员聚类，但与衣原体属、衣原体属、Simkania 属和 Waddlia 属的谱系不同（序列相似性，
The recently proposed reorganization of the order Chlamydiales and description of new taxa are broadening our perception of this once narrowly defined taxon. We have recovered four strains of gram-negative cocci endosymbiotic in Acanthamoeba spp,, representing 5% of the Acanthamoeba sp. isolates examined, which displayed developmental life cycles typical of members of the Chlamydiales, One of these endosymbiont strains was found stably infecting an amoebic isolate recovered from a case of amoebic keratitis in North America, with three others found in acanthamoebae recovered from environmental sources in North America (two isolates) and Europe (one isolate). Analyses of nearly full-length 16S rRNA gene sequences of these isolates by neighbor joining, parsimony, and distance matrix methods revealed their clustering with other members of the Chlamydiales but in a lineage separate from those of the genera Chlamydia, Chlamydophila, Simkania, and Waddlia (sequence similarities,