Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells
Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells
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DOI：
10.1111/j.1365-2591.2006.01097.x
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发表时间：
2006-05-01
影响因子：
5
通讯作者：
Ito, K
中科院分区：
文献类型：
--
作者：
Takita, T;Hayashi, M;Ito, K
目的探讨三氧化矿物聚集体（mineral trioxide aggregate，MTA）对人牙髓细胞（Human dental pulp，HDP）体外增殖的影响。对照细胞仅用培养板插入物培养。使用细胞计数试剂盒测量细胞增殖长达14天，并使用钙E测试试剂盒评估从测试材料释放的钙离子浓度。为了确认MTA的效果是由于释放的钙离子，细胞增殖测定在存在外源性氯化钙作为钙离子的来源，而在没有MTA.Results三氧化矿物质聚集体显着刺激细胞增殖12天后，而Dycal没有这样的效果。MTA释放的钙离子数量显著高于Dycal。加入氯化钙后，12天后细胞增殖以剂量依赖性方式增加。此外，细胞增殖表现出相似的模式是否产生一定浓度的钙离子由氯化钙或释放从MTA.结论在这个离体研究中，洗脱成分，如MTA的钙离子有较高的HDP细胞增殖能力比对照和Dycal。
Aim To investigate the effect of mineral trioxide aggregate (MTA) on the proliferation of human dental pulp (HDP) cells ex-vivo.Methodology Human dental pulp cells were cultured with MTA or calcium hydroxide-containing cement (Dycal) using culture plate inserts. Control cells were cultured with culture plate inserts only. Cell proliferation was measured for up to 14 days using a Cell Counting kit, and the concentration of calcium ions released from the tested materials was assessed using a Calcium E-test kit. To confirm that the effect of MTA was attributable to released calcium ions, cell proliferation was measured in the presence of exogenous calcium chloride as a source of calcium ions while in the absence of MTA.Results Mineral trioxide aggregate significantly stimulated cell proliferation after 12 days, whereas Dycal had no such effect. The number of calcium ions released from MTA was significantly higher than that released from Dycal. Following the addition of calcium chloride, cell proliferation increased in a dose-dependent manner after 12 days. Moreover, cell proliferation showed a similar pattern whether a given concentration of calcium ions was produced by calcium chloride or by release from MTA.Conclusions In this ex-vivo study, the elution components such as calcium ions from MTA had higher proliferation ability of HDP cells than control and Dycal.