NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer Part II. The preparation of a molecular ruler
NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer Part II. The preparation of a molecular ruler
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DOI：
10.1016/j.chemphyslip.2008.07.007
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发表时间：
2008-10-01
影响因子：
3.4
通讯作者：
Frimer, Aryeh A.
中科院分区：
文献类型：
--
作者：
Cohen, Yael;Afri, Michal;Frimer, Aryeh A.
我们先前已经示出了如何通过使用可极化碳的C-13 NMR化学位移（例如，羰基或硝酰基碳）和该碳所在的微环境的极性（使用Dimroth-Reichardt的E-T（30）参数）。在一篇配套论文中，我们确定了报告分子的标准，这将有助于我们将定性极性数据转换为定量埃值。在本论文中，我们报告了我们在DMPC双层定量映射中的初步成功，通过距离（以埃为单位）和E-T（30）极性连接双层内的两个或多个垂直点。结果与欧文伦敦的“视差法”得到的值相关性很好。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
We have previously shown how the location of an intercalant within the lipid bilayer can be qualitatively determined by using the excellent correlation that exists between the C-13 NMR chemical shift of a polarizable carbon (e.g., the carbonyl or nitronyl carbon) and the polarity (using the Dimroth-Reichardt's E-T(30) parameter) of the microenvironment in which that carbon resides. In a companion paper, we have determined criteria for reporter molecules that will assist us in converting this qualitative polarity data into quantitative Angstrom values. In the present paper, we report on our initial success in quantitatively mapping of the DMPC bilayer by linking two or more vertical points within a bilayer by both distance (in Angstroms) and E-T(30) polarity. The results correlated well with the values obtained using the "parallax method" of Erwin London. (C) 2008 Elsevier Ireland Ltd. All rights reserved.