Fas and Fas ligand: strong co‐expression in human hepatocytes surrounding hepatocellular carcinoma; can cancer induce suicide in peritumoural cells?
Fas and Fas ligand: strong co‐expression in human hepatocytes surrounding hepatocellular carcinoma; can cancer induce suicide in peritumoural cells?
复制标题
Fas 和 Fas 配体：在肝细胞癌周围的人肝细胞中强烈共表达；癌症会诱导瘤周细胞自杀吗？
作者：
T. Roskams;L. Libbrecht;Boudewijn Van Damme;Valeer Desmet
Fas（Apo-1，CD 95）是神经生长因子/肿瘤坏死因子受体超家族的成员，响应激动性抗体或Fas配体（Fas-L）结合介导细胞凋亡。Fas已被证明存在于正常肝脏和慢性丙型肝炎的肝细胞膜上。目前，关于Fas‐L表达的数据非常有限。本文描述了一项对20例不同病因的活动性慢性肝炎、20例肝细胞癌（HCC）和邻近非肿瘤肝实质以及5例正常肝脏的研究。使用特异性单克隆抗体测定Fas和Fas‐L的免疫组织化学表达。在正常肝脏中，Fas在肝细胞和胆管细胞膜上微弱表达，而Fas‐L为阴性。在活动性慢性肝炎，Fas在肝细胞中的表达增强，导致弥漫性蜂窝图案。Fas‐L在界面肝炎区域的肝细胞中显示胞浆阳性。在紧邻HCC的肝细胞中Fas和Fas‐L的强表达是一个恒定的发现。在HCC中，Fas‐L表达是可变的，但仅存在于少数细胞中。Fas变化从弥漫性蜂窝状图案局灶性阳性，偶尔细胞。肿瘤中Fas和Fas‐L表达之间没有相关性。总之，肝细胞可以在界面肝炎和HCC附近区域共表达Fas和Fas‐L，表明它们具有以自分泌或旁分泌方式诱导细胞凋亡的能力。在肿瘤内，Fas-Fas-L凋亡途径似乎很少参与。版权所有© 2000约翰威利父子有限公司。
Fas (Apo‐1, CD95), a member of the nerve growth factor/tumour necrosis factor receptor superfamily, mediates apoptosis in response to agonistic antibodies or Fas ligand (Fas‐L) binding. Fas has been shown to be present on hepatocyte membranes in normal liver and in chronic hepatitis C. At the present time, very limited data are available on the expression of Fas‐L. This paper describes a study of 20 cases of active chronic hepatitis of different aetiologies, 20 hepatocellular carcinomas (HCCs) and the adjacent non‐tumoural liver parenchyma, and five normal livers. The immunohistochemical expression of Fas and Fas‐L was determined using specific monoclonal antibodies. In normal liver, Fas was faintly expressed on membranes of hepatocytes and bile duct cells, while Fas‐L was negative. In active chronic hepatitis, Fas expression in hepatocytes was enhanced, resulting in a diffuse honeycomb pattern. Fas‐L showed cytoplasmic positivity in hepatocytes in areas of interface hepatitis. Strong expression of Fas as well as Fas‐L in the hepatocytes immediately adjacent to HCC was a constant finding. Within the HCCs, Fas‐L expression was variable, but present only in a minority of cells. Fas varied from a diffuse honeycomb pattern to focal positivity in occasional cells. There was no correlation between Fas and Fas‐L expression in the tumours. In conclusion, hepatocytes can co‐express Fas and Fas‐L in areas of interface hepatitis and adjacent to HCC, suggesting that they have the ability to induce apoptosis in an autocrine or paracrine way. Within the tumour, the Fas–Fas‐L apoptosis pathway seems to be little involved. Copyright © 2000 John Wiley & Sons, Ltd.