Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation.
Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation.
复制标题
人牙周膜干细胞成骨分化过程中的动态蛋白质组学分析
DOI：
10.1186/s13287-020-02123-6
复制
发表时间：
2021-02-03
影响因子：
7.5
通讯作者：
Qiu W
中科院分区：
文献类型：
--
作者：
Li J;Wang Z;Huang X;Wang Z;Chen Z;Wang R;Chen Z;Liu W;Wu B;Fang F;Qiu W
人牙周膜干细胞是牙周组织再生的理想种子细胞。深入了解成骨分化过程中蛋白质的动态变化有助于牙周组织工程再生的研究。Tandem Mass Tag定量蛋白质组学用于揭示hPDLSC在成骨分化过程中第0、3、7和14天的时间蛋白质表达模式。差异表达蛋白（DEP）进行聚类和功能注释的基因本体论（GO）术语。基于京都基因和基因组百科全书数据库进行途径富集分析，然后使用Incidity Pathway Analysis软件进行预测的活化。氧化还原敏感的信号转导途径和氧化磷酸化（OXPHOS）的相互作用网络进行了验证与蛋白质印迹的枢纽蛋白SOD 2。共有1024个DEP被确定和聚类在5个不同的集群代表动态趋势。GO富集结果表明，具有不同倾向的蛋白质表现出不同的功能。途径富集分析发现OXPHOS显著参与，这进一步预测了持续激活。具有动态激活状态的氧化还原敏感信号通路显示出与OXPHOS不同程度的关联，特别是sirtuin信号通路。SOD 2是沉默调节蛋白途径的重要组成部分，在骨生成过程中表现出持续增加。数据可通过ProteomeXchange获得，标识符为PXD 020908。这是第一个深入的动态蛋白质组学分析的hPDLSCs的成骨分化。这为牙周组织再生的研究提供了新的视角。在线版本包含补充材料，可通过10.1186/s13287-020-02123-6获得。
Human periodontal ligament stem cells (hPDLSCs) are ideal seed cells for periodontal regeneration. A greater understanding of the dynamic protein profiles during osteogenic differentiation contributed to the improvement of periodontal regeneration tissue engineering. Tandem Mass Tag quantitative proteomics was utilized to reveal the temporal protein expression pattern during osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) were clustered and functional annotated by Gene Ontology (GO) terms. Pathway enrichment analysis was performed based on the Kyoto Encyclopedia of Genes and Genomes database, followed by the predicted activation using Ingenuity Pathway Analysis software. Interaction networks of redox-sensitive signalling pathways and oxidative phosphorylation (OXPHOS) were conducted and the hub protein SOD2 was validated with western blotting. A total of 1024 DEPs were identified and clustered in 5 distinctive clusters representing dynamic tendencies. The GO enrichment results indicated that proteins with different tendencies show different functions. Pathway enrichment analysis found that OXPHOS was significantly involved, which further predicted continuous activation. Redox-sensitive signalling pathways with dynamic activation status showed associations with OXPHOS to various degrees, especially the sirtuin signalling pathway. SOD2, an important component of the sirtuin pathway, displays a persistent increase during osteogenesis. Data are available via ProteomeXchange with identifier PXD020908. This is the first in-depth dynamic proteomic analysis of osteogenic differentiation of hPDLSCs. It demonstrated a dynamic regulatory mechanism of hPDLSC osteogenesis and might provide a new perspective for research on periodontal regeneration. The online version contains supplementary material available at 10.1186/s13287-020-02123-6.
登录
查看更多内容
影响因子：
2.8
作者：
Case, Natasha;Xie, Zhihui;Sen, Buer;Styner, Maya;Zou, Minxu;O'Conor, Chris;Horowitz, Mark;Rubin, Janet
通讯作者：
Rubin, Janet
影响因子：
4.9
作者：
Abed É;Delalandre A;Lajeunesse D
通讯作者：
Lajeunesse D
影响因子：
6.2
作者：
Bivi, Nicoletta;Condon, Keith W.;Allen, Matthew R.;Farlow, Nathan;Passeri, Giovanni;Brun, Lucas R.;Rhee, Yumie;Bellido, Teresita;Plotkin, Lilian I.
通讯作者：
Plotkin, Lilian I.
影响因子：
11.4
作者：
Brent, Michael R.
通讯作者：
Brent, Michael R.
影响因子：
16.6
作者：
Fu, Rong;Lv, Wen-Cong;Wu, Zhao-Qiu
通讯作者：
Wu, Zhao-Qiu