pGreen:: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation
pGreen:: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation
复制标题
DOI：
10.1023/a:1006496308160
复制
发表时间：
2000-04-01
影响因子：
5.1
通讯作者：
Mullineaux, PM
中科院分区：
文献类型：
--
作者：
Hellens, RP;Edwards, EA;Mullineaux, PM
二元Ti载体是农杆菌介导的植物转化方案中选择的质粒载体。pGreen系列二元Ti载体的配置是为了易于使用，并满足许多植物物种的广泛转化程序的需求。该质粒系统允许选择标记和报告基因在左右T-DNA边界的任何排列，而不损害克隆限制性位点的选择，因为pGreen克隆位点基于众所周知的pBluescript通用载体质粒。其在大肠杆菌中的大小和拷贝数为常规体外重组程序提供了更高的效率。只有当另一个质粒pSoup在同一菌株中共存时，pGreen才能在农杆菌中复制。pSoup为pGreen提供了反式的复制功能。RepA和Mob函数的删除使得pGreen的大小保持在最小。pGreen的版本已用于转化几种植物物种，其效率与其他二元Ti载体相同。pGreen质粒系统的信息由因特网网站（http：//www.example.com）补充，通过该网站可以找到不同pGreen标记基因排列的综合信息、方案、订单和列表。www.pgreen.ac.uk
Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of selectable marker and reporter gene at the right and left T-DNA borders without compromising the choice of restriction sites for cloning, since the pGreen cloning sites are based on the well-known pBluescript general vector plasmids. Its size and copy number in Escherichia coli offers increased efficiencies in routine in vitro recombination procedures. pGreen can replicate in Agrobacterium only if another plasmid, pSoup, is co-resident in the same strain. pSoup provides replication functions in trans for pGreen. The removal of RepA and Mob functions has enabled the size of pGreen to be kept to a minimum. Versions of pGreen have been used to transform several plant species with the same efficiencies as other binary Ti vectors. Information on the pGreen plasmid system is supplemented by an Internet site (http://www.pgreen.ac.uk) through which comprehensive information, protocols, order forms and lists of different pGreen marker gene permutations can be found.