BACILLUS-THURINGIENSIS DELTA-ENDOTOXIN EXPRESSED IN TRANSGENIC NICOTIANA-TABACUM PROVIDES RESISTANCE TO LEPIDOPTERAN INSECTS
BACILLUS-THURINGIENSIS DELTA-ENDOTOXIN EXPRESSED IN TRANSGENIC NICOTIANA-TABACUM PROVIDES RESISTANCE TO LEPIDOPTERAN INSECTS
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DOI：
10.1104/pp.85.4.1103
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发表时间：
1987-12-01
期刊：
影响因子：
7.4
通讯作者：
YANG, NS
中科院分区：
文献类型：
--
作者：
BARTON, KA;WHITELEY, HR;YANG, NS
晶体蛋白，或δ-苏云金芽孢杆菌的内毒素对鳞翅目昆虫是特异性致死的。我们利用克隆的晶体蛋白基因的截短和修饰部分来构建能够在植物细胞中表达的嵌合基因。利用根癌农杆菌双元载体系统，我们将嵌合毒素基因转入烟草（Nicotiana tabacum cv Havana 425）细胞并再生重组植株。重组植物中通常存在每个细胞一至几个拷贝的毒素基因。杂交实验表明，这些植物有一个新的RNA物种的大小预期的截短的毒素mRNA，和多肽具有预期的移动性截短的毒素通过免疫印迹检测。不同重组体之间的毒素特异性RNA表达水平存在显著差异，但转化体中的杂交RNA水平与对烟草天蛾（烟草天蛾）和其他鳞翅目昆虫表现出的毒性水平相关。将重组基因传递给后代并保持对昆虫的抗性，从而证明将毒素基因导入植物中可能是提供对某些昆虫害虫的保护的实用方法。
The crystal proteins, or .delta.-endotoxins, of Bacillus thuringiensis are specifically lethal to Lepidopteran insects. We utilized a truncated and modified portion of a cloned crystal protein gene to construct a chimeric gene capable of expression in plant cells. Using an Agrobacterium tumefaciens binary vector system, we then transferred the chimeric toxin gene into tobacco (Nicotiana tabacum cv Havana 425) cells and regenerated recombinant plants. One to several copies per cell of the toxin gene are routinely present in the recombinant plants. Hybridization experiments demonstrated that these plants had a new RNA species of the size expected for the truncated toxin mRNA, and a polypeptide having the mobility expected for the truncated toxin was detected by immunoblotting. Significant variation was found in the levels of toxin-specific RNA expression between different recombinants, but the levels of hybridizing RNA in transformants correlated with the level of toxicity demonstrated against Manduca sexta (tobacco hornworm), and other Lepidopteran insects. The recombinant genes were transmitted to progeny and resistance to insects was maintained, thus demonstrating that the introduction of toxin genes into plants may be a practical method of providing protection against certain insect pests.