Toll Like Receptor 4 (Tlr4) And p65 Nuclear Factor Kappa B (Nf-Kb) Expression In Monocyte Cell Of Children With Steroid Resistant Nephrotic Syndrome
Toll Like Receptor 4 (Tlr4) And p65 Nuclear Factor Kappa B (Nf-Kb) Expression In Monocyte Cell Of Children With Steroid Resistant Nephrotic Syndrome
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类固醇抵抗性肾病综合征患儿单核细胞中 Toll 样受体 4 (Tlr4) 和 p65 核因子 Kappa B (Nf-Kb) 的表达
DOI：
10.11594/jtls.04.02.01
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发表时间：
2014
影响因子：
--
通讯作者：
A. Endharti
中科院分区：
文献类型：
--
作者：
T. Wahyono;Krisni Subandiyah;L. E. Fitri;A. Endharti
本研究旨在证明激素敏感性肾病综合征（SSNS）患儿与激素抵抗性肾病综合征（SRNS）患儿TLR4和p65NF-kB亚基表达的差异，并探讨SRNS患儿TLR4和p65NF-kB表达的相关性。 23 名特发性肾病综合征 (INS) 患者纳入本研究，并分为两组：SSNS 组（11 名患者）和 SRNS 组（12 名患者）。从 5 ml 患者血液样本中提取外周血单核细胞 (PBMC)，然后使用单核细胞培养物分离 PMBC。使用流式细胞术检查单核细胞培养物中的 TLR4 和 p65NF-kB 表达。结果显示，SSNS和SRNS之间TLR4表达差异有显着性(p=0.012)，SSNS和SRNS之间p65 NF-kB表达差异无显着相关性(p=0.880)。 TLR4与p65NF-kB表达之间的Pearson相关性检验显示无显着相关性(p=0.472)，而TLR4与SRNS表达之间关系的Spearman相关性检验得出的相关值为0.512，其重要性p值为0.013(p<0.05)。 SRNS和SSNS之间TLR4的表达存在显着差异，且TLR4与SRNS的表达也存在显着相关性，证明TLR4在肾病综合征的激素抵抗中具有重要作用。
This research aimed to prove the difference of TLR4 and p65NF-kB subunit expression between children with steroid-sensitive nephrotic syndrome (SSNS) and those with steroid-resistent nephrotic syndrome (SRNS) and to find out the correlation between expression of TLR4 and p65NF-kB on children with SRNS. Twenty three patients of idiopathic nephrotic syndrome patients (INS) were enrolled in this study and classified into two groups—those with SSNS (11 patients) and SRNS (12 patients). Peripheral Blood Mononuclear Cells (PBMC) isolation was performed, taken from 5 ml patients’ blood samples, followed by monocyte isolation of PMBC using monocyte cultu re. Examination of TLR4 and p65NF-kB expression from monocyte's cells culture was performed using flow cytometry. The result showed that the differences of TLR4 expression between SSNS and SRNS was significant (p=0.012), The differences of p65 NF-kB expression between SSNS and SRNS showed an insignificant correlation (p=0.880). Pearson correlation test between the expression of TLR4 and p65NF-kB showed no significant correlation (p=0.472), while Spearman correlation test to see the relationship between expression of TLR4 and SRNS produced correlation value of 0.512 and its importance p value was 0.013 (p<0.05). There is a significant difference of TLR4 expression between SRNS and SSNS and there is also a significant correlation between expression of TLR4 and SRNS, that proved TLR4 has an important role in steroid resistance of nephrotic syndrome.
影响因子：
14.2
作者：
Li, LB;Goleva, E;Leung, DYM
通讯作者：
Leung, DYM