The effects of the morphine analogue levorphanol on leukocytes. Metabolic effects at rest and during phagocytosis.
The effects of the morphine analogue levorphanol on leukocytes. Metabolic effects at rest and during phagocytosis.
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吗啡类似物左啡诺对白细胞的影响。
DOI：
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发表时间：
1971
影响因子：
15.9
通讯作者：
S. Lebow
中科院分区：
文献类型：
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作者：
N. Wurster;P. Elsbach;E. J. Simon;P. Pettis;S. Lebow
对细菌的研究表明，吗啡类药物对细胞膜有影响。为了确定这类药物对哺乳动物细胞的影响，我们选择了兔腹膜渗出液粒细胞，它在吞噬过程中发生了显着的膜变化。我们研究了体外吗啡类似物，左啡烷对吞噬作用和代谢的粒细胞孵育和没有聚苯乙烯颗粒或活大肠杆菌的影响。左啡烷（1或2毫摩尔/升）减少：（a）培养基中溶血磷脂酰乙醇胺或溶血磷脂酰乙醇胺的酰化（其在吞噬作用期间被刺激约两倍）在静止（40%）和在吞噬作用期间（60%）;（B）乳胶颗粒和大肠杆菌的摄取，如通过电子显微镜所判断的;（c）活大肠杆菌的杀死（10倍）;（d）（14）CO（2）在吞噬作用期间从葡萄糖-1-（14）C产生至少80%;（e）粒细胞的K（+）含量（35%）;（f）亚油酸-1-（14）C氧化50%，并且其并入甘油三酯中超过80%。然而，左啡烷刺激2至3倍的亚油酸-1-（14）C或棕榈酸-1-（14）C掺入几种磷脂。葡萄糖摄取、乳酸产生和三磷酸腺苷（ATP）含量不受药物影响。因此，左啡烷似乎不通过全身代谢抑制发挥其作用。通过两次重悬粒细胞去除左啡烷完全逆转所有抑制。与对细菌的观察一致，似乎左啡烷对粒细胞的复杂影响可能至少部分是由于对细胞膜的影响。
Studies on bacteria have suggested that morphine-like drugs have effects on the cell membrane. To determine the effect of this class of drugs on a mammalian cell, we selected the rabbit peritoneal exudate granulocyte, which undergoes striking membrane changes during phagocytosis. We examined the effect in vitro of the morphine analogue, levorphanol on phagocytosis and metabolism by granulocytes incubated with and without polystyrene particles or live Escherichia coli. Levorphanol (1 or 2 mmoles/liter) decreased: (a) acylation of lysolecithin or lysophosphatidylethanolamine in the medium (which is stimulated about two-fold during phagocytosis) both at rest (40%) and during phagocytosis (60%); (b) uptake of latex particles and Escherichia coli, as judged by electron microscopy; (c) killing of live Escherichia coli (10-fold); (d) (14)CO(2) production from glucose-1-(14)C during phagocytosis by at least 80%; (e) K(+) content of granulocytes (35%); (f) oxidation of linoleate-1-(14)C by 50%, and its incorporation into triglyceride by more than 80%. However, levorphanol stimulated 2 to 3-fold the incorporation of linoleate-1-(14)C or palmitate-1-(14)C into several phospholipids. Glucose uptake, lactate production, and adenosine triphosphate (ATP) content are not affected by the drug. Thus, levorphanol does not appear to exert its effects through generalized metabolic suppression. Removal of levorphanol by twice resuspending the granulocytes completely reverses all inhibition. In line with observations on bacteria, it appears that the complex effects of levorphanol on granulocytes may be due at least in part to an effect on the cell membrane.