Molecularly imprinted polymers based on multi-walled carbon nanotubes for selective solid-phase extraction of oleanolic acid from the roots of kiwi fruit samples.
Molecularly imprinted polymers based on multi-walled carbon nanotubes for selective solid-phase extraction of oleanolic acid from the roots of kiwi fruit samples.
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DOI：
10.1016/j.talanta.2012.07.066
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发表时间：
2012-09
期刊：
影响因子：
6.1
通讯作者：
Xing Chen;Zhaohui Zhang;Xiao Yang;Jiaxing Li;Yunan Liu;Hongjun Chen;Wei Rao;S. Yao
中科院分区：
文献类型：
--
作者：
Xing Chen;Zhaohui Zhang;Xiao Yang;Jiaxing Li;Yunan Liu;Hongjun Chen;Wei Rao;S. Yao
以油酸为模板，4-乙烯基吡啶为功能单体，二乙烯基苯为交联剂，采用热诱导聚合法合成了新型多壁碳纳米管分子印迹聚合物（MWNTs@MIPs）。采用傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）对MWNTs@MIPs进行了表征。详细研究了MWNTs@MIPs对齐墩果酸的吸附过程。对MWNTs@MIPs的固相萃取性能进行了评价。结果表明，MWNTs@MIPs具有良好的印迹效果和相当的选择性。采用优化的分子印迹固相萃取法从猕猴桃根提取物中提取油酸。猕猴桃样品中油酸的加标回收率在84-92.6%之间，相对标准偏差小于5%，检出限为2.56μgL−1。
This study describes the synthesis of novel molecularly imprinted polymers based on multi-walled carbon nanotubes (MWNTs@MIPs) using oleanolic acid as the template, 4-vinylpyridine as the functional monomer and divinylbenzene as the cross-linker by heat-induced polymerization. The MWNTs@MIPs were characterized with Fourier transform infrared (FT-IR) spectrometry and scanning electron microscopy (SEM). The adsorption process of the MWNTs@MIPs towards oleanolic acid was investigated in detail. The properties of MWNTs@MIPs for solid-phase extraction (SPE) were also evaluated. The results demonstrated the good imprinting effect and the comparable selectivity of MWNTs@MIPs. The optimized molecularly imprinted solid-phase extraction (MISPE) procedure was applied to extract oleanolic acid from the extracts of the roots of kiwi fruit samples. The recoveries of spiked oleanolic acid in kiwi fruit samples were in the range of 84–92.6% with relative standard deviations below 5%, and its limit of detection reached 2.56μgL−1.