Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress
Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress
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DOI：
10.1016/j.plaphy.2012.10.012
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发表时间：
2013-01-01
影响因子：
6.5
通讯作者：
Du, Yu-Guang
中科院分区：
文献类型：
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作者：
Liu, Hang;Zhang, Yun-Hong;Du, Yu-Guang
由海藻酸盐降解制备的海藻酸盐寡糖（AOS）是一种有效的植物引发剂。通过水培实验，探讨AOS提高小麦抗干旱能力的机制。通过将小麦根部暴露于聚乙二醇-6000（PEG-6000）溶液（150 g·L-1）4天来模拟干旱模型，经PEG处理的小麦生长显着下降。而施用AOS后，脱水状态下小麦的幼苗和根长、鲜重和相对含水量与PEG组相比分别增加了18%、26%、43%和33%。此外，经AOS处理的样品抗氧化酶活性明显增强，丙二醛（MDA）含量降低37.9%。此外，参与ABA信号通路的抗旱相关基因，如胚胎发生晚期丰度蛋白1基因（LEA1）、psbA基因、蔗糖非发酵1相关蛋白激酶2基因（SnRK2）和吡咯啉-5-羧酸合成酶基因（P5CS）均被AOS上调。我们的研究结果表明，AOS 可能通过调节 ABA 依赖性信号通路来增强小麦生育期的干旱胁迫抗性。 (C) 2012 Elsevier Masson SAS。版权所有。
Alginate oligosaccharides (AOS) prepared from degradation of alginate is a potent plant elicitor. Hydroponic experiments were carried out to investigate the mechanism of AOS on improving Triticum aestivum L resistant ability to drought stress. Drought model was simulated by exposing the roots of wheat to polyethylene glycol-6000 (PEG-6000) solution (150 g L-1) for 4 days and the growth of wheat treated with PEG was significantly decreased. However, after AOS application, seedling and root length, fresh weight and relative water content of wheat were increased by 18%, 26%, 43% and 33% under dehydration status compared with that of PEG group, respectively. Moreover, the antioxidative enzymes activities were obviously enhanced and malondialdehyde (MDA) content was reduced by 37.9% in samples treated by AOS. Additionally, the drought resistant related genes involved in ABA signal pathway, such as late embryogenesis abundant protein 1 gene (LEA1), psbA gene, Sucrose non-fermenting 1-related protein kinase 2 gene (SnRK2) and Pyrroline-5-Carboxylate Synthetase gene (P5CS) were up-regulated by AOS. Our results suggested that AOS might regulate ABA-dependent signal pathway to enhance drought stress resistance of wheat during growth period. (C) 2012 Elsevier Masson SAS. All rights reserved.