Hydrogel contact lens-induced Pseudomonas keratitis in a rabbit model.
Hydrogel contact lens-induced Pseudomonas keratitis in a rabbit model.
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水凝胶隐形眼镜引起的兔模型中的假单胞菌角膜炎。
DOI：
10.1097/00003226-198706040-00005
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发表时间：
1987
期刊：
影响因子：
2.8
通讯作者：
Kenyon,KR
中科院分区：
文献类型：
--
作者：
Durán,JA;Refojo,MF;Kenyon,KR
将含有38.6％水(polymacon)和70％水(lidofilcon A)的未使用的软性隐形眼镜(SCL)暴露于铜绿假单胞菌(10 8 CFU/ml)60分钟并洗涤。在以下条件下将受污染的镜片用于兔子眼睛：在划伤的角膜上皮上使用1小时（16只眼睛，4只感染），在正常角膜上使用24小时（8只眼睛，无感染），以及在正常角膜上使用7天（8只眼睛，3只细菌感染和2只非感染上皮缺损）。对照组兔子使用未污染的镜片 7 天（八只眼睛，四只未感染的上皮缺陷）。对照组和佩戴受污染镜片 24 小时和 7 天的组进行睑板缝合术以保持隐形眼镜就位。此外，将3滴细菌悬浮液滴入正常角膜上皮眼（8只眼，无感染）和划伤角膜上皮眼（16只眼，13只感染）。结果表明，假单胞菌污染的 SCL 仅在受损的角膜上皮上引起角膜炎，并且细菌悬浮液对兔角膜的致病性比 SCL 上类似数量的细菌更具致病性。
Unused soft contact lenses (SCL) containing 38.6% water (polymacon) and 70% water (lidofilcon A) were exposed to Pseudomonas aeruginosa (10 8 CFU/ml) for 60 min and washed. The contaminated lenses were used on rabbit eyes under the following conditions: for 1 h on a scratched corneal epithelium (16 eyes, four infected), for 24 h on normal cornea (eight eyes, none infected), and for 7 days on normal cornea (eight eyes, three bacterial infections and two noninfected epithelial defects). A control group of rabbits used an uncontaminated lens for 7 days (eight eyes, four noninfected epithelial defects). The control group and groups that wore contaminated lenses for 24 h and 7 days underwent tarsorrhaphy to keep the contact lens in place. In addition, three drops of the bacterial suspension were instilled in eyes with normal corneal epithelium (eight eyes, none infected) and scratched corneal epithelium (16 eyes, 13 infected). Results suggest that Pseudomonas-contaminated SCL give rise to keratitis only on an injured corneal epithelium and that a bacterial suspension is more pathogenic to the rabbit cornea than is a similar amount of bacteria on an SCL.