Differential expression of Tet family genes and their potential role in regulating skeletal muscle development of Siniperca chuatsi
Differential expression of Tet family genes and their potential role in regulating skeletal muscle development of Siniperca chuatsi
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DOI：
10.1016/j.aqrep.2023.101486
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发表时间：
2023-04
影响因子：
3.7
通讯作者：
Jia Hua Cheng;Xin Zhu;Yangyang Meng;Congyi Cheng;Wei Zeng;Lin Chen;Yaxiong Pan;W. Chu;
中科院分区：
文献类型：
--
作者：
Jia Hua Cheng;Xin Zhu;Yangyang Meng;Congyi Cheng;Wei Zeng;Lin Chen;Yaxiong Pan;W. Chu;
DNA甲基化和去甲基化是动物发育过程中重要的表观遗传修饰和调控，其动态变化可能影响骨骼肌的发育。10 - 11易位（泰特）家族蛋白是参与DNA甲基化动态变化的脱甲基酶。然而，Tetfamily基因的表达模式及其在鱼类肌肉发生中的作用尚不清楚。本研究采用RT-qPCR技术分析了Tet 1、Tet 2和Tet 3基因在中华鲟中的时空表达谱。结果表明，Tet 1在囊胚期低表达，在原肠胚期高表达，之后一直低表达到孵化。Tet 2和Tet 3在原肠胚晚期表达量显著增加，并在孵化前一直保持高表达。在空间表达水平上，Tet 1在鳃组织中表达量最高，在脑和慢肌中表达量中等; Tet 2在慢肌中表达量较低，与Tet 1相似; Tet 3在鳃和脑中表达量较高，在快肌中表达量中等。余弦分析结果表明，Tet 1和Tet 2在快肌中的表达具有明显的日节律性，而Tet 3在快肌中的表达没有明显的日节律性。注射Bobcat 339抑制Tet 1/2蛋白的活性可显著降低MyoD和MRF 4的表达，但对MyoGandMyf 5的表达无影响，从而导致卫星细胞和增殖的成肌细胞数量增加。这些结果提示Tet 1/2可能靶向MyoD 4和MRF 4，导致DNA去甲基化并促进其表达，从而刺激成肌细胞分化。
DNA methylation and demethylation are crucial epigenetic modification and regulation for animal development, and their dynamic changes may affect skeletal muscle development. The ten-eleven translocation (Tet) family proteins are demethylases which are involved in the dynamic changes of DNA methylation. However, the expression pattern ofTetfamily genes and their role in myogenesis in fish remains unclear. In this study, the temporal and spatial expression profiles ofTet1,Tet2andTet3were assayed with RT-qPCR techniques in Chinese perch,Siniperca chuatsi. The obtained data showed that threeTetfamily genes were differentially expressed at different development stages.Tet1was expressed low at blastula stage, but highly expressed at gastrula stage, then remained low until hatching. The expressions ofTet2andTet3were significantly increased at late gastrula and kept high expression before hatching stage. At the spatial level, theTet1expression was highest in gill tissue, moderate level in brain and slow muscle.Tet2was similar to that ofTet1except that it was expressed at a lower level in slow muscle, andTet3exhibited a higher expression level in gill and brain, a moderate level in fast muscle. Cosinor analysis turned out that the expression ofTet1andTet2displayed a significant daily rhythm in fast muscle, butTet3did not show daily rhythmicity. Inhibiting the activity of Tet1/2 proteins by injecting Bobcat339 significantly reduced the expression ofMyoDandMRF4, but notMyoGandMyf5, by which leads to the increase of the number of satellite cells and proliferating myoblasts. Together, the results suggest that Tet1/2 may target toMyoDandMRF4resulted in DNA demethylation and promoting their expression, thus stimulating myoblast differentiation.