Increased expression of CENP-H gene in human oral squamous cell carcinomas harboring high-proliferative activity.
Increased expression of CENP-H gene in human oral squamous cell carcinomas harboring high-proliferative activity.
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DOI：
10.3892/or.16.5.1071
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发表时间：
2006-11
期刊：
影响因子：
4.2
通讯作者：
H. Shigeishi;K. Higashikawa;Shigehiro Ono;K. Mizuta;Yoshiaki Ninomiya;Shingo Yoneda;M. Taki;N. Kamata
中科院分区：
文献类型：
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作者：
H. Shigeishi;K. Higashikawa;Shigehiro Ono;K. Mizuta;Yoshiaki Ninomiya;Shingo Yoneda;M. Taki;N. Kamata
应用实时荧光定量逆转录-聚合酶链反应（RT-PCR）检测了38例口腔鳞状细胞癌（SCC）、2例上皮不典型增生和5例正常牙龈组织中着丝粒蛋白H（CENP-H）mRNA的表达。CENP-H mRNA的平均表达水平在口腔SCC中（0.11+/-0.08）高于上皮发育不良（0.03+/-0.01）和正常牙龈炎（0.027+/-0.01）。CENP-H mRNA在口腔鳞癌中的表达水平显著高于正常牙龈组织（Mann-Whitney U检验，P=0.005）。我们还发现CENP-H mRNA表达水平与口腔SCC的临床分期之间存在显著相关性（Mann-Whitney U检验，P=0.04）。接下来，我们用化学方法研究了CENP-H在17个口腔SCC中的表达。CENP-H蛋白的表达水平与Ki-67标记指数之间存在显著相关性（Mann-Whitney U检验，P=0.005）。这些结果表明，人CENP-H与恶性疾病中增加的或异常的细胞增殖密切相关。
We examined the expression of Centromere protein H (CENP-H) mRNA in 38 oral squamous cell carcinomas (SCCs), 2 epithelial dysplasias and 5 normal gingivae using the real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). The mean expression level of CENP-H mRNA was higher in oral SCCs (0.11+/-0.08) than epithelial dysplasias (0.03+/-0.01) and normal gingivae (0.027+/-0.01). The expression level of CENP-H mRNA was significantly higher in oral SCCs than normal gingivae (Mann-Whitney U test, P=0.005). We also found a significant association between the level of CENP-H mRNA expression and clinical stage in oral SCCs (Mann-Whitney U test, P=0.04). We next studied the expression of CENP-H in 17 oral SCCs immunohistochemically. A significant correlation between the expression levels of CENP-H protein and the Ki-67 labeling index was found (Mann-Whitney U test, P=0.005). These results indicate that human CENP-H is closely linked to the increased or abnormal cell proliferation in malignant conditions.