Isolation and characterization of microsatellite loci in tiger frog (Hoplobatrachus rugulosus)

Isolation and characterization of microsatellite loci in tiger frog (Hoplobatrachus rugulosus)
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DOI:
10.1007/s10592-008-9803-y
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发表时间:
2009-01
影响因子:
2.2
通讯作者:
Chen Shao;Yu Wang;N. Qiao
Chen Shao;Yu Wang;N. Qiao
中科院分区:
环境科学与生态学3区
文献类型:
--
作者:
Chen Shao;Yu Wang;N. Qiao

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利用富集技术从虎纹蛙(Hoplobatrachus rugulosus)中开发了9个新的微卫星位点。等位基因数为6 ~ 11个,平均每个位点8.56个。观察杂合度为0.571 ~ 0.935(平均0.796),期望杂合度为0.765 ~ 0.888(平均0.837)。这些多态位点可以用于群体结构、基因流和群体分化。
Nine novel microsatellite loci were developed from the tiger frog (Hoplobatrachus rugulosus) using enrichment methods. The number of alleles ranged from 6 to 11 with an average of 8.56 alleles per locus. The observed and expected heterozygosity ranged from 0.571 to 0.935 (average 0.796) and 0.765 to 0.888 (average 0.837) respectively. These polymorphic loci can be used in population structure, gene flow, and population differentiation.