Metabolic energy reduction by glucose deprivation and low gas exchange preserves platelet function after 48 h storage at 4 °C
Metabolic energy reduction by glucose deprivation and low gas exchange preserves platelet function after 48 h storage at 4 °C
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DOI：
10.1111/j.1423-0410.2007.00891.x
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发表时间：
2007-01-01
期刊：
影响因子：
2.7
通讯作者：
Pietersz, R. N. I.
中科院分区：
文献类型：
--
作者：
Badlou, B. A.;van der Meer, P. F.;Pietersz, R. N. I.
前言：我们早些时候的研究表明，在37℃的无糖、抗霉素A介质中孵育制备的代谢抑制的血小板(MSP)在4℃下的持续保存效果好于22℃下未经处理的对照组。然而，使用线粒体抑制剂抗霉素A不能用于血小板输注。目的研究如何在没有抗霉素A的情况下制备能量降低(ER)的血小板。研究设计与方法将放在无葡萄糖介质中的气体不透气袋中的血小板在22℃保存4h以减少能量储存，然后在4℃(ER22-4)保存。结果保存48h后，ER22-4在pH保存(6.4+/-0.4vs.5.0+/-0.4，n=4)、血小板计数(800+/-225vs.800+/-225vs.650+/-150×10(9))，凝血酶受体激活肽诱导的聚集(50+/-15比10+/-5%)和糖蛋白Ibα表达(60+/-15比28+/-15)。ER22-4细胞中GPIBα的表达高于ER4细胞，说明能量抑制在冷藏过程中保存了GPIBα。结论在不使用抗霉素A的情况下，将血小板保存在不透气的袋子中，可以模拟代谢抑制。能量抑制在4℃储存过程中保存了GPIBα的表达。
Introduction We showed earlier that metabolically suppressed platelets (MSP) prepared by incubation in glucose-free, antimycin A medium at 37 degrees C better sustained storage at 4 degrees C than untreated controls at 22 degrees C. However, the use of the mitochondrial inhibitor antimycin A is incompatible with platelet transfusion.Objectives The aim of this study was to investigate how energy-reduced (ER) platelets could be prepared in the absence of antimycin A.Study Design and Methods Platelets in gas-impermeable bags in glucose-free medium were kept at 22 degrees C for 4 h to reduce energy stores and thereafter stored at 4 degrees C (ER22-4). Controls were energy-reduced platelets without prior incubation at 22 degrees C (ER4), and MSPs in test tubes and untreated platelets in gas-permeable bags with glucose and stored at 22 degrees C (C22) and 4 degrees C (C4).Results After 48 h storage, ER22-4 were superior to C22 with respect to pH preservation (6.4 +/- 0.4 vs. 5.0 +/- 0.4, n = 4),platelet count (800 +/- 225vs. 650 +/- 150 x 10(9)), thrombin receptor-activating peptide-induced aggregation (50 +/- 15 vs. 10 +/- 5%) and glycoprotein (GP)Ib alpha expression (60 +/- 15% vs. 28 +/- 15). GPIb alpha expression was higher in ER22-4 than in ER4, indicating that energy suppression preserved GPIb alpha during cold storage.Conclusions Metabolic suppression without the use of antimycin A could be mimicked by storage of platelets in glucose-free medium in gas-impermeable bags. Energy suppression preserved GPIb alpha expression during storage at 4 degrees C.