Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds
Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds
复制标题
DOI：
10.1089/10763270050199550
复制
发表时间：
2000-12-01
影响因子：
--
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Shea, LD;Wang, D;Mooney, DJ
骨再生是基于这样的假设：健康的祖细胞，无论是被招募还是被运送到受伤部位，最终都能再生丢失或受损的组织。三维多孔聚合物支架可以通过创造和维持促进祖细胞迁移、增殖和分化的空间来增强骨再生。作为测试这种可能性的第一步，成骨细胞在由可生物降解聚合物制成的支架上培养，并评估这些支架上的骨发育情况。以生物可降解的丙交酯和乙醇酯聚合物为原料制备多孔聚合物支架。将MC3T3-E1细胞静态接种到聚合物支架上，在抗坏血酸和磷酸甘油酯的作用下体外培养，细胞在培养的前4周内增殖并形成填充组织。在培养过程中，这些细胞中的胶原信使RNA水平一直很高，这与观察到的聚合物支架上胶原沉积的增加是一致的。沉积的胶原矿化最初在4周观察到，随后增加。矿化的发生与两种成骨细胞特异性基因：骨钙素和骨唾液蛋白的mRNA水平升高相对应。细胞/聚合物构建体培养12周后，形成了与天然骨结构相似的三维组织。这些研究表明，三维工程组织中的成骨细胞遵循二维培养中描述的经典分化途径。诸如此类的聚合物支架可能最终用于临床，通过向伤口部位输送或募集祖细胞来增强骨再生。
Bone regeneration is based on the hypothesis that healthy progenitor cells, either recruited or delivered to an injured site, can ultimately regenerate lost or damaged tissue. Three-dimensional porous polymer scaffolds may enhance bone regeneration by creating and maintaining a space that facilitates progenitor cell migration, proliferation, and differentiation. As an initial step to test this possibility, osteogenic cells were cultured on scaffolds fabricated from biodegradable polymers, and bone development on these scaffolds was evaluated. Porous polymer scaffolds were fabricated from biodegradable polymers of lactide and glycolide. MC3T3-E1 cells were statically seeded onto the polymer scaffolds and cultured in vitro in the presence of ascorbic acid and P-glycerol phosphate, The cells proliferated during the first 4 weeks in culture and formed a space-filling tissue. Collagen messenger RNA levels remained high in these cells throughout the time in culture, which is consistent with an observed increase in collagen deposition on the polymer scaffold. Mineralization of the deposited collagen was initially observed at 4 weeks and subsequently increased. The onset of mineralization corresponded to increased mRNA levels for two osteoblast-specific genes: osteocalcin and bone sialoprotein. Culture of cell/polymer constructs for 12 weeks led to formation of a three-dimensional tissue with architecture similar to that of native bone. These studies demonstrate that osteoblasts within a three-dimensional engineered tissue follow the classic differentiation pathway described for two-dimensional culture. Polymer scaffolds such as these may ultimately be used clinically to enhance bone regeneration by delivering or recruiting progenitor cells to the wound site.