Evolutionary fate of rhizome-specific genes in a non-rhizomatous Sorghum genotype
Evolutionary fate of rhizome-specific genes in a non-rhizomatous Sorghum genotype
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DOI：
10.1038/hdy.2008.119
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发表时间：
2009-03-01
期刊：
影响因子：
3.8
通讯作者：
Paterson, A. H.
中科院分区：
文献类型：
--
作者：
Jang, C. S.;Kamps, T. L.;Paterson, A. H.
器官丢失后，器官特异性基因的命运如何？对于先前被证明具有根茎富集表达的Sorcenpropinquum和Sorcenhalepense基因，我们对Sorcenbicolor（非根茎性）和S.pinquum的编码区和调控序列进行了比较分析。根状茎（rhizomatousness）。大多数富含根茎表达的基因在两种基因型中似乎具有相似的旁系同源拷贝数，研究的24个基因中只有3个在拷贝数上显示出显着差异。我们在S中没有检测到更大的突变倾向。bicolor比S.在后者中具有根茎富集表达的基因的propinquum。几个顺式作用调控元件，特别是一个Myb结合核心（AACGG），参与有丝分裂细胞周期蛋白的调控，在S. propinquum比非根状茎S. bicolor或Oryza sativa（水稻）。我们认为，许多基因的根茎富集表达在S。propinquum可能具有多种功能，在S.双色，但正在进行的净化选择，以保持其余的功能。多倍体S. halepense根茎似乎更频繁地来自S. propinquum比S.双色祖先，但有一些证据表明，形成新的等位基因和“招募”的S。bicolor基因在S. halepense，提示多倍体可能为S.哈利潘塞
What is the fate of organ-specific genes after the organ is lost? For Sorghum propinquum and Sorghum halepense genes that were previously shown to have rhizome-enriched expression, we have conducted comparative analysis of both coding regions and regulatory sequences in Sorghum bicolor (non-rhizomatousness) and S. propinquum (rhizomatousness). Most genes with rhizome-enriched expression appear to have similar numbers of paralogous copies in both genotypes, with only three of 24 genes studied showing significant differences in copy numbers. We detected no greater propensity for mutation in S. bicolor than in S. propinquum of genes with rhizome-enriched expression in the latter. Several cis-acting regulatory elements, particularly an Myb-binding core (AACGG) that is involved in the regulation of the mitotic cyclin, were more abundant in promoters of S. propinquum than in non-rhizomatous S. bicolor or Oryza sativa ( rice). We suggest that many genes with rhizome-enriched expression in S. propinquum may serve multiple functions, with partial loss of some of these functions in S. bicolor but ongoing purifying selection acting to preserve the remaining functions. Expressed genes in polyploid S. halepense rhizomes appeared to be more frequently derived from the S. propinquum than the S. bicolor progenitor, but there was some evidence of formation of novel alleles and 'recruitment' of S. bicolor genes to rhizome-enriched expression in S. halepense, suggesting that polyploidy may have offered new evolutionary potential to S. halepense.