The stromal cell-derived factor-1α dependent migration of human cord blood CD34+ haematopoietic stem and progenitor cells switches from protein kinase C (PKC)-α dependence to PKC-α independence upon prolonged culture in the presence of Flt3-ligand and interleukin-6
The stromal cell-derived factor-1α dependent migration of human cord blood CD34+ haematopoietic stem and progenitor cells switches from protein kinase C (PKC)-α dependence to PKC-α independence upon prolonged culture in the presence of Flt3-ligand and interleukin-6
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DOI：
10.1111/j.1365-2141.2008.07256.x
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发表时间：
2008-09-01
影响因子：
6.5
通讯作者：
Dittmar, Thomas
中科院分区：
文献类型：
--
作者：
Kasenda, Benjamin;Kassmer, Susannah H.;Dittmar, Thomas
向人脐带血造血干细胞和祖细胞（HSPCs）培养基中添加炎性细胞因子白细胞介素(IL)-6已被证明可导致基质细胞衍生因子-1 α依赖性迁移表型的改变。这项研究调查了这种影响是否归因于蛋白激酶C （PKC）同种型的不同参与。flt3 -配体和flt3 -配体/IL-6培养的脐带血造血干细胞在第1天的迁移活性均依赖于pkc - α，但在第5天的培养后不依赖于pkc - α。在培养5 d的细胞中，pkc - α的表达没有下调，这表明指示细胞迁移的信号分子发生了改变。
Addition of the inflammatory cytokine interleukin (IL)-6 to the culture medium of human cord blood haematopoietic stem and progenitor cells (HSPCs) has been shown to lead to an altered stromal cell-derived factor-1 alpha-dependent migratory phenotype. This study investigated whether this effect was attributed to a differential engagement of protein kinase C (PKC) isotypes. The migratory activity of both Flt3-ligand and Flt3-ligand/IL-6 cultured cord blood HSPCs was PKC-alpha dependent on day 1, but PKC-alpha independent after 5 d of cultivation. PKC-alpha expression was not down-regulated in cells cultured for 5 d indicating a switch of signalling molecules directing cell migration.