THE INFLUENCE OF PHOSPHOLIPASE-A2 AND GLUTATHIONE-PEROXIDASE ON THE ELIMINATION OF MEMBRANE LIPID PEROXIDES
THE INFLUENCE OF PHOSPHOLIPASE-A2 AND GLUTATHIONE-PEROXIDASE ON THE ELIMINATION OF MEMBRANE LIPID PEROXIDES
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DOI：
10.1016/0003-9861(83)90608-2
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发表时间：
1983-01-01
影响因子：
3.9
通讯作者：
MONTESTRUQUE, S
中科院分区：
文献类型：
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作者：
SEVANIAN, A;MUAKKASSAHKELLY, SF;MONTESTRUQUE, S
膜脂过氧化产物的释放和磷脂酶的作用之间的关系进行了研究。大鼠肝微粒体和磷脂酰胆碱脂质体-磷脂酶A2制剂进行抗坏血酸铁诱导的脂质过氧化反应。过氧化产物的特点是通过测量丙二醛和脂质过氧化物。实验的目的是证明磷脂酶依赖的去除过氧化产物起源于膜。增加溶血磷脂酰胆碱的形成是明显的脂质过氧化作用后，磷脂酶A2的脂质体，这是抑制剂对溴苯甲酰甲基溴和米帕林。脂氧合酶依赖的耗氧量，以及过氧化物从微粒体转移到孵育介质，在很大程度上依赖于磷脂酶，并可以减少磷脂酶抑制剂。此外，脂质过氧化氢形成的磷脂酰胆碱脂质体铁抗坏血酸诱导的过氧化反应，或那些存在于老化的脂质体，有效地减少谷胱甘肽过氧化物酶时，磷脂酶A2存在于测定。低水平的谷胱甘肽过氧化物酶的活性，观察磷脂酶A2的情况下。
The relationship between release of membrane lipid peroxidation products and phospholipase action was examined. Rat liver microsomes and phosphatidylcholine liposome-phospholipase A2 preparations were subjected to iron ascorbate-induced lipid peroxidation. Peroxidation products were characterized by measurement of malondialdehyde and lipid peroxides. Experiments were designed to demonstrate phospholipase-dependent removal of peroxidation products originating in the membrane. Increased lysophosphatidylcholine formation was evident following lipid peroxidation in phospholipase A2-containing liposomes which was inhibitor by p-bromophenacyl bromide and mepacrine. Lipoxygenase-dependent oxygen consumption, as well as peroxide transfer from microsomes to the incubation medium, was largely dependent on phospholipase and could be diminished by phospholipase inhibitors. Furthermore, lipid hydroperoxides formed by subjecting phosphatidylcholine liposomes to iron ascorbate-induced peroxidation, or those present in aged liposomes, were effectively reduced by glutathione peroxidase when phospholipase A2 was present in the assay. Low level glutathione peroxidase activity was observed in the absence of phospholipase A2.