CELL-MIGRATION AND INDUCTION IN THE DEVELOPMENT OF THE SURFACE ECTODERMAL PATTERN OF THE XENOPUS-LAEVIS TADPOLE
CELL-MIGRATION AND INDUCTION IN THE DEVELOPMENT OF THE SURFACE ECTODERMAL PATTERN OF THE XENOPUS-LAEVIS TADPOLE
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DOI：
10.1111/j.1440-169x.1992.00051.x
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发表时间：
1992-02-01
影响因子：
2.5
通讯作者：
ELINSON, RP
中科院分区：
文献类型：
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作者：
DRYSDALE, TA;ELINSON, RP
非洲爪蟾的表面含有三种特殊的瞬时细胞类型：纤毛细胞、孵化腺细胞和水泥腺细胞。为了区分这些细胞类型在表面的出现是由于表面细胞的诱导还是由于深层外胚层细胞迁移到表面，我们将标记的表面或深层细胞移植到原肠胚早期到中期的未标记宿主中。将宿主培养成蝌蚪后（第 28 阶段），我们检查了胚胎表面的纤毛细胞、孵化腺细胞和骨水泥腺细胞，并观察了哪些细胞被标记。我们发现所有纤毛细胞与其他功能未知的细胞一起从深层外胚层移至表面。与大多数水泥腺细胞一样，孵化腺细胞是通过表面细胞的诱导而产生的。一些深层细胞产生水泥腺细胞。因此，深层细胞向表面的迁移和表面细胞的局部诱导有助于非洲爪蟾蝌蚪的最终表面图案化。
The surface of theXenopustadpole contains three specialized, transient cell types; the ciliated, hatching gland, and cement gland cells. To distinguish whether the appearance of these cell types on the surface is due to induction of surface cells or due to migration of deep ectodermal cells into the surface, we transplanted labelled surface or deep cells to unlabelled hosts at early to mid‐gastrulae. After raising the host to a tadpole (Stage 28), we examined the embryo's surface for ciliated, hatching gland, and cement gland cells, and observed which cells were labelled. We find that all ciliated cells move into the surface from the deep ectodermal layer along with other cells of unknown function. Hatching gland cells arise by induction of surface cells as do the majority of cement gland cells. A few deep cells give rise to cement gland cells. Therefore, migration of deep cells to the surface and localized induction of surface cells contribute to the final surface patterning of theXenopustadpole.