Dopamine stimulates somatostatin release from perifused hypothalamic cells.
Dopamine stimulates somatostatin release from perifused hypothalamic cells.
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多巴胺刺激灌注的下丘脑细胞释放生长抑素。
DOI：
10.1152/ajpendo.1983.244.6.e560
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Hollander,CS
中科院分区：
文献类型：
--
作者：
Richardson,SB;Nguyen,T;Hollander,CS
我们研究了免疫反应性生长抑素（IR-SRIF）的释放从大鼠下丘脑细胞，获得和分散的援助与胶原酶。分散后24小时，将细胞置于由预溶胀生物凝胶P2基质支撑的柱中并灌流。在冰上收集馏分，随后测定SRIF。钾去极化（KCl，56 mM）、Na+-K+-ATP酶抑制剂哇巴因（10（-4）M）和低至10（-11）M的多巴胺显著刺激SRIF释放。膜去极化的刺激作用是钙依赖性的，并且在灌流介质中不存在外源性钙或存在EDTA（0.05 M）的情况下未观察到。多巴胺拮抗剂甲氧氯普胺可阻断多巴胺的兴奋作用。总之，可以在简单的灌流装置中研究分散的大鼠下丘脑细胞释放IR-SRIF。释放是由膜去极化以钙依赖的方式和多巴胺在生理浓度刺激。
We studied the release of immunoreactive somatostatin (IR-SRIF) from hypothalamic cells that were obtained from rats and dispersed with the aid of collagenase. Twenty-four hours after dispersion, cells were placed in a column supported by a matrix of preswollen Biogel P2 and perifused. Fractions were collected on ice and subsequently assayed for SRIF. SRIF release was stimulated markedly by potassium depolarization (KCl, 56 mM), by the Na+-K+-ATPase inhibitor ouabain (10(-4) M), and by dopamine at concentrations as low as 10(-11) M. The stimulatory effects of membrane depolarization were calcium dependent and were not observed in the absence of exogenous calcium in the perifusion medium or in the presence of EDTA (0.05 M). Metoclopramide, the dopamine antagonist, abolished the stimulatory effect of dopamine. In conclusion, release of IR-SRIF by dispersed rat hypothalamic cells can be studied in a simple perifusion apparatus. Release is stimulated by membrane depolarization in a calcium-dependent manner and by dopamine at physiological concentrations.