Relationships Between Copper-Related Proteomes and Lifestyles in β Proteobacteria
Relationships Between Copper-Related Proteomes and Lifestyles in β Proteobacteria
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DOI：
10.3389/fmicb.2019.02217
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发表时间：
2019-09-24
影响因子：
5.2
通讯作者：
Jacob-Dubuisson, Francoise
中科院分区：
文献类型：
--
作者：
Antoine, Rudy;Rivera-Millot, Alex;Jacob-Dubuisson, Francoise
铜是一种重要的过渡金属，其氧化还原特性可用于各种酶催化氧化还原和电子转移链中。它对生物也是有毒的，因此它的细胞浓度必须严格控制。我们对100多种贝塔蛋白细菌的预测蛋白质组进行了电子分析，以确定它们与铜相关的蛋白质组，包括铜蛋白，即具有活性中心铜离子的蛋白质，铜伴侣蛋白和铜稳态系统。与铜相关的蛋白质组占b变形杆菌总蛋白质组的0-1.48%。CuProteins的数量与所有系统发育类群中的蛋白质组大小成全球比例，并与有氧呼吸密切相关。相比之下，环境细菌的铜稳态系统的比例比其他细菌组大得多，无论它们的蛋白质组大小如何。向共生性、专性、宿主限制的致病或共生的进化在全球范围内反映在铜稳态系统的丧失上。在内共生体中，防御系统和铜伴侣已经消失，而残留的铜酶是有氧呼吸的电子转移蛋白。因此，生活方式是铜相关蛋白质组大小和组成的主要决定因素，尤其反映在涉及铜稳态的系统中。对伯克霍尔德氏菌、波尔德氏菌和奈瑟氏菌属一些物种与铜有关的蛋白质组的分析表明，共生体正在脱离其铜稳态系统和伴侣蛋白的过程中，但比病原体更大的程度。
Copper is an essential transition metal whose redox properties are used for a variety of enzymatic oxido-reductions and in electron transfer chains. It is also toxic to living beings, and therefore its cellular concentration must be strictly controlled. We have performed in silico analyses of the predicted proteomes of more than one hundred species of beta proteobacteria to characterize their copper-related proteomes, including cuproproteins, i.e., proteins with active-site copper ions, copper chaperones, and copper-homeostasis systems. Copper-related proteomes represent between 0 and 1.48% of the total proteomes of b proteobacteria. The numbers of cuproproteins are globally proportional to the proteome sizes in all phylogenetic groups and strongly linked to aerobic respiration. In contrast, environmental bacteria have considerably larger proportions of copper-homeostasis systems than the other groups of bacteria, irrespective of their proteome sizes. Evolution toward commensalism, obligate, host-restricted pathogenesis or symbiosis is globally reflected in the loss of copper-homeostasis systems. In endosymbionts, defense systems and copper chaperones have disappeared, whereas residual cuproenzymes are electron transfer proteins for aerobic respiration. Lifestyle is thus a major determinant of the size and composition of the copper-related proteome, and it is particularly reflected in systems involved in copper homeostasis. Analyses of the copper-related proteomes of a number of species belonging to the Burkholderia, Bordetella, and Neisseria genera indicates that commensals are in the process of shedding their copper-homeostasis systems and chaperones to greater extents yet than pathogens.