A Novel Silver Bioactive Glass Elicits Antimicrobial Efficacy Against Pseudomonas aeruginosa and Staphylococcus aureus in an ex Vivo Skin Wound Biofilm Model.
A Novel Silver Bioactive Glass Elicits Antimicrobial Efficacy Against Pseudomonas aeruginosa and Staphylococcus aureus in an ex Vivo Skin Wound Biofilm Model.
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DOI：
10.3389/fmicb.2018.01450
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发表时间：
2018
影响因子：
5.2
通讯作者：
Hardman MJ
中科院分区：
文献类型：
--
作者：
Wilkinson HN;Iveson S;Catherall P;Hardman MJ
生物膜感染现在被认为是慢性伤口愈合的一个重要因素。细菌生物膜逃避宿主的免疫反应，并显示出对抗生素的耐药性增加。沿着抗生素管理的改进，迫切需要有效的新的抗生物膜疗法来有效地进行伤口管理。先前的研究表明，生物活性玻璃（Bg）能够促进愈合，具有适度的杀菌活性。在这里，我们测试了一种新的BG结合银（BgAg）的抗菌功效，对创伤相关细菌铜绿假单胞菌和金黄色葡萄球菌的渗透和生物膜形式。在时间杀灭试验（2小时内细菌活力降低6个对数）和琼脂扩散试验中，BgAg稳定、持久，对嗜热菌有效。在生理学相关的离体猪伤口生物膜模型中，BgAg降低了细菌负荷;铜绿假单胞菌（2-log降低）和S.金黄色葡萄球菌（3-log减少）。BgAg还对铜绿假单胞菌生物膜毒力产生强烈影响，降低蛋白酶活性和毒力基因表达。共培养生物膜对BgAg的抗性更强，其中S.金黄色葡萄球菌。最后，BgAg被证明有利于宿主对生物膜感染的反应，直接减少宿主组织细胞死亡。总之，这些发现提供了证据，表明BgAg对嗜热菌和单一物种生物膜具有强效抗菌作用，对宿主组织反应具有有益作用。需要进一步研究以阐明BG给药对多微生物生物膜的具体后果，并进一步探索对宿主-微生物相互作用的影响。
Biofilm infection is now understood to be a potent contributor to the recalcitrant nature of chronic wounds. Bacterial biofilms evade the host immune response and show increased resistance to antibiotics. Along with improvements in antibiotic stewardship, effective new anti-biofilm therapies are urgently needed for effective wound management. Previous studies have shown that bioactive glass (Bg) is able to promote healing with moderate bactericidal activity. Here we tested the antimicrobial efficacy of a novel BG incorporating silver (BgAg), against both planktonic and biofilm forms of the wound-relevant bacteria Pseudomonas aeruginosa and Staphylococcus aureus. BgAg was stable, long lasting, and potently effective against planktonic bacteria in time-kill assays (6-log reduction in bacterial viability within 2 h) and in agar diffusion assays. BgAg reduced bacterial load in a physiologically relevant ex vivo porcine wound biofilm model; P. aeruginosa (2-log reduction) and S. aureus (3-log reduction). BgAg also conferred strong effects against P. aeruginosa biofilm virulence, reducing both protease activity and virulence gene expression. Co-culture biofilms appeared more resistant to BgAg, where a selective reduction in S. aureus was observed. Finally, BgAg was shown to benefit the host response to biofilm infection, directly reducing host tissue cell death. Taken together, the findings provide evidence that BgAg elicits potent antimicrobial effects against planktonic and single-species biofilms, with beneficial effects on the host tissue response. Further investigations are required to elucidate the specific consequences of BG administration on polymicrobial biofilms, and further explore the effects on host–microbe interactions.