Silencing long non-coding RNA DLX6-AS1 or restoring microRNA-193b-3p enhances thyroid carcinoma cell autophagy and apoptosis via depressing HOXA1.
Silencing long non-coding RNA DLX6-AS1 or restoring microRNA-193b-3p enhances thyroid carcinoma cell autophagy and apoptosis via depressing HOXA1.
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DOI：
10.1111/jcmm.16868
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发表时间：
2021-10
影响因子：
5.3
通讯作者：
Fang J
中科院分区：
文献类型：
--
作者：
Feng L;Wang R;Wang Y;Shen X;Shi Q;Lian M;Ma H;Fang J
长非编码RNA DLX6反义RNA1(DLX6-AS1)在甲状腺癌(TC)的发生发展中具有重要地位。然而，对DLX6-AS1、microRNA(MiR)-193B-3p和同源盒A1(HOXA1)在TC中的整体理解还不够彻底。鉴于此，本研究以DLX6-AS1/miR-193B-3p/HOXA1为轴心，研究了DLX6-AS1/miR-193B-3p/HOXA1在TC细胞生长和自噬中的作用。收集甲状腺癌组织和癌旁正常甲状腺组织，检测DLX6-AS1、miR-193B-3p和HOXA1的表达及其相互作用。将DLX6-AS1/miR-193B-3p相关的寡核苷酸或质粒导入TC细胞，检测细胞生长和自噬能力。观察裸鼠体内的成瘤情况。在TC中，Dlx6-AS1和HOXA1表达上调，miR-193B-3p表达下调。去除DLX6-AS1或修复miR-193B-3p干扰细胞生长并促进自噬。Dlx6-AS1靶向miR-193B-3p并正调控HOXA1。MIR-193B-3P抑制可减轻DLX6-AS1下调所致的肿瘤形成障碍。裸鼠体内的致瘤性与细胞内的致瘤性一致。很明显，DLX6-AS1缺失通过上调miR-193B-3p和下调HOXA1来阻碍TC细胞的生长并促进自噬。
Long non‐coding RNA DLX6 antisense RNA 1 (DLX6‐AS1) lists a critical position in thyroid carcinoma (TC) development. However, the overall comprehension about DLX6‐AS1, microRNA (miR)‐193b‐3p and homeobox A1 (HOXA1) in TC is not thoroughly enough. Concerning to this, this work is pivoted on DLX6‐AS1/miR‐193b‐3p/HOXA1 axis in TC cell growth and autophagy. TC tissues and adjacent normal thyroid tissues were collected, in which expression of DLX6‐AS1, miR‐193b‐3p and HOXA1 was tested, together with their interactions. TC cells were transfected with DLX6‐AS1/miR‐193b‐3p‐related oligonucleotides or plasmids to test cell growth and autophagy. Tumorigenesis in nude mice was observed. DLX6‐AS1 and HOXA1 were up‐regulated, and miR‐193b‐3p was down‐regulated in TC. Depleted DLX6‐AS1 or restored miR‐193b‐3p disturbed cell growth and promoted autophagy. DLX6‐AS1 targeted miR‐193b‐3p and positively regulated HOXA1. miR‐193b‐3p inhibition mitigated the impaired tumorigenesis induced by down‐regulated DLX6‐AS1. Tumorigenesis in nude mice was consistent with that in cells. It is clear that DLX6‐AS1 depletion hinders TC cell growth and promotes autophagy via up‐regulating miR‐193b‐3p and down‐regulating HOXA1.
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