IGF-1 and Its Receptor in Esophageal Cancer: Association with Adenocarcinoma and Visceral Obesity
IGF-1 and Its Receptor in Esophageal Cancer: Association with Adenocarcinoma and Visceral Obesity
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DOI：
10.1038/ajg.2011.417
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发表时间：
2012-02-01
影响因子：
9.8
通讯作者：
Lysaght, Joanne
中科院分区：
文献类型：
--
作者：
Doyle, Suzanne L.;Donohoe, Claire L.;Lysaght, Joanne
结论：胰岛素样生长因子（IGF）途径和内脏肥胖与食管癌独立相关。本研究旨在探讨内脏性肥胖与IGF-1系统在食管腺癌和食管鳞癌中的差异和相互联系。方法：采用Western blotting方法检测食管鳞癌（KYSE 410，OE 21）和食管腺癌（OE 19，OE 33）细胞系IGF-1受体（IGF-1 R）mRNA和蛋白表达。通过溴脱氧尿苷掺入试验评估肿瘤细胞对IGF-1的增殖反应。在食管肿瘤切片中，通过免疫组化评估IGF-1 R表达和CD 68（+）细胞数量。采用酶联免疫吸附法检测食管癌患者、Barrett食管患者和健康对照者血清中IGF-1的含量。结果：食管鳞癌细胞中IGF-1 R蛋白表达高于食管腺癌细胞，但只有食管腺癌细胞系对IGF-1有明显的增殖反应（P
OBJECTIVES: The insulin-like growth factor (IGF) pathway and visceral obesity have been independently linked with esophageal cancer. This study aimed to delineate the differential and interlinked role of visceral obesity and the IGF-1 system in esophageal adenocarcinoma and esophageal squamous-cell carcinoma (SCC).METHODS: IGF-1 receptor (IGF-1R) mRNA and protein were examined in esophageal SCC (KYSE 410, OE21) and esophageal adenocarcinoma (OE19, OE33) cell lines by western blotting. Tumor cell proliferation in response to IGF-1 was assessed by bromodeoxyuridine incorporation assay. In esophageal tumor sections, expression of IGF-1R and CD68(+) cell numbers were assessed by immunohistochemistry. IGF-1 was measured in serum from esophageal cancer patients, Barrett's esophagus patients, and healthy controls by enzyme-linked immunosorbent assay.RESULTS: Higher IGF-1R protein expressions were observed in SCC cells compared with esophageal adenocarcinoma cells however only adenocarcinoma cell lines significantly increased proliferation in response to IGF-1 (P