Myosin-1C associates with microtubules and stabilizes the mitotic spindle during cell division
Myosin-1C associates with microtubules and stabilizes the mitotic spindle during cell division
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DOI：
10.1242/jcs.084335
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发表时间：
2011-08-01
影响因子：
4
通讯作者：
Tsiavaliaris, Georgios
中科院分区：
文献类型：
--
作者：
Rump, Agrani;Scholz, Tim;Tsiavaliaris, Georgios
真核细胞中的有丝分裂纺锤体由微管（MT）和相关蛋白的双极阵列组成，所述微管和相关蛋白在有丝分裂期间需要用于将两组染色体正确分配到子细胞。除了MT相关蛋白（MAP）和MT马达在细胞分裂中的既定功能外，越来越多的证据表明，基于F-肌动蛋白的肌球蛋白马达是有丝分裂期间F-肌动蛋白-MT相互作用的重要介质。在这里，我们报告的长尾类-1肌球蛋白肌球蛋白-1C有丝分裂过程中的有丝分裂。我们的数据表明，肌球蛋白-1C结合到MT，并在维持纺锤体稳定性的准确染色体分离的作用。肌球蛋白-1C马达功能和尾域介导的MT-F-肌动蛋白相互作用都是蛋白质从细胞外周到纺锤体的细胞周期依赖性重新定位所必需的。我们发现，肌球蛋白-1C与MT的协会是通过尾部结构域介导的。肌球蛋白-1C尾可以抑制驱动蛋白的运动活性，增加肌球蛋白的稳定性，并在肌球蛋白和F-肌动蛋白之间形成交联。这些数据表明，肌球蛋白-1C参与了有丝分裂过程中MT功能的调节。盘状突
The mitotic spindle in eukaryotic cells is composed of a bipolar array of microtubules (MTs) and associated proteins that are required during mitosis for the correct partitioning of the two sets of chromosomes to the daughter cells. In addition to the well-established functions of MT-associated proteins (MAPs) and MT-based motors in cell division, there is increasing evidence that the F-actin-based myosin motors are important mediators of F-actin-MT interactions during mitosis. Here, we report the functional characterization of the long-tailed class-1 myosin myosin-1C from Dictyostelium discoideum during mitosis. Our data reveal that myosin-1C binds to MTs and has a role in maintenance of spindle stability for accurate chromosome separation. Both myosin-1C motor function and tail-domain-mediated MT-F-actin interactions are required for the cell-cycle-dependent relocalization of the protein from the cell periphery to the spindle. We show that the association of myosin-1C with MTs is mediated through the tail domain. The myosin-1C tail can inhibit kinesin motor activity, increase the stability of MTs, and form crosslinks between MTs and F-actin. These data illustrate that myosin-1C is involved in the regulation of MT function during mitosis in D. discoideum.