Effects of inhibitors of protein synthesis on the ovulatory process of the perfused rat ovary.
Effects of inhibitors of protein synthesis on the ovulatory process of the perfused rat ovary.
复制标题
蛋白质合成抑制剂对灌注大鼠卵巢排卵过程的影响。
DOI：
10.1530/jrf.0.0850451
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
K. Ahrén
中科院分区：
文献类型：
--
作者：
M. Brännström;B. Boberg;J. Törnell;P. Janson;K. Ahrén
采用体外灌注大鼠卵巢模型，研究了两种不同的蛋白质合成抑制剂（环己亚胺和嘌呤霉素）对体外排卵过程的影响。灌注20 iu PMSG大鼠卵巢21 h，测定环腺苷3′，5′-单磷酸腺苷（cAMP）和甾体激素（孕酮、睾酮和雌二醇）的释放量，计数释放的卵母细胞数估算排卵数。未受刺激的对照卵巢不排卵，而添加黄体生成素（0.1微克/毫升）和3-异丁基-1-甲基黄嘌呤（IBMX; 0.2 mM）后，每个处理卵巢排卵16.7 +/- 3.5次。环己亚胺（5微克/毫升）在开始灌注和LH + IBMX后8小时加入时均能完全抑制LH + IBMX的排卵作用。在LH + IBMX后10 h（第一次排卵前1-1.5 h，每个处理卵巢15.2 +/- 4.4次排卵）加入环己亚胺无抑制作用。Puromycin（200微克/毫升）从灌注开始就完全阻断排卵，当LH + IBMX后8小时加入该化合物时，抑制率达到60%（每个处理卵巢6.5 +/- 2.2次排卵）。两种抑制剂均显著增加了LH + ibmx刺激的cAMP释放，但降低了黄体酮、睾酮和雌二醇的释放。这些结果表明，在卵泡破裂发生的排卵过程中，de-novo蛋白的合成是重要的。
The effects of two different protein synthesis inhibitors (cycloheximide and puromycin) on the ovulatory process were examined in vitro using a perfused rat ovary model. Ovaries of PMSG (20 i.u.)-primed rats were perfused for 21 h. Release of cyclic adenosine 3',5'-monophosphate (cAMP) and steroids (progesterone, testosterone, and oestradiol) was measured and the number of ovulations was estimated by counting released oocytes. Unstimulated control ovaries did not ovulate whereas addition of LH (0.1 microgram/ml) plus 3-isobutyl-1-methylxanthine (IBMX; 0.2 mM) resulted in 16.7 +/- 3.5 ovulations per treated ovary. Cycloheximide (5 micrograms/ml) totally inhibited the ovulatory effect of LH + IBMX when present from the beginning of the perfusions and also when added 8 h after LH + IBMX. No inhibition was seen when cycloheximide was added 10 h after LH + IBMX (1-1.5 h before the first ovulation; 15.2 +/- 4.4 ovulations per treated ovary). Puromycin (200 micrograms/ml) completely blocked ovulation when present from the beginning of the perfusions and the inhibition was congruent to 60% (6.5 +/- 2.2 ovulations per treated ovary) when the compound was added 8 h after LH + IBMX. Both inhibitors increased LH + IBMX-stimulated cAMP release substantially, but decreased the release of progesterone, testosterone and oestradiol. These results indicate that de-novo protein synthesis is important late in the ovulatory process for follicular rupture to occur.
抑制雌激素合成不会抑制黄体生成素诱导的排卵。
影响因子：
9.8
作者：
Koos,RD;Feiertag,MA;Brodie,AM;LeMaire,WJ
通讯作者：
LeMaire,WJ
放线菌酮抑制兔卵巢卵泡中的排卵、前列腺素生物合成和类固醇生成。
DOI：
10.1530/jrf.0.0780679
发表时间：
1986
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Espey,LL
通讯作者：
Espey,LL
放线菌酮对 rRNA、5S RNA 和 tRNA 基因转录的影响。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gokal,PK;Cavanaugh,AH;ThompsonJr,EA
通讯作者：
ThompsonJr,EA