Peptide methionine sulfoxide reductase (MsrA) is a virulence determinant in Mycoplasma genitalium
Peptide methionine sulfoxide reductase (MsrA) is a virulence determinant in Mycoplasma genitalium
复制标题
DOI：
10.1128/jb.183.19.5645-5650.2001
复制
发表时间：
2001-10-01
影响因子：
3.2
通讯作者：
Baseman, JB
中科院分区：
文献类型：
--
作者：
Dhandayuthapani, S;Blaylock, MW;Baseman, JB
生殖支原体是最小的自我复制微生物，并与人类疾病有关，包括泌尿生殖道和呼吸道感染以及关节炎。四.生殖器主要通过由表面相关膜蛋白（包括粘附素和细胞粘附相关蛋白）网络介导的粘附机制来定殖宿主细胞。在本文中，我们表明，细胞粘附在M。生殖器受称为肽甲硫氨酸亚砜还原酶（MsrA）的不相关蛋白质影响，MsrA是一种催化蛋白质中的甲硫氨酸亚砜[Met（O）]残基还原为甲硫氨酸的抗氧化剂修复酶。对M.通过同源重组构建的生殖器显示出对绵羊红细胞的粘附显著降低。此外，msrA突变体不能在仓鼠中生长，并且与野生型毒性M.生殖器这些结果表明MsrA在M.生殖器致病性，可能是通过保护支原体蛋白结构免受氧化损伤或通过替代的毒力相关途径。
Mycoplasma genitalium is the smallest self-replicating microorganism and is implicated in human diseases, including urogenital and respiratory infections and arthritides. IV. genitalium colonizes host cells primarily through adherence mechanisms mediated by a network of surface-associated membrane proteins, including adhesins and cytadherence-related proteins. In this paper, we show that cytadherence in M. genitalium is affected by an unrelated protein known as peptide methionine sulfoxide reductase (MsrA), an antioxidant repair enzyme that catalyzes the reduction of methionine sulfoxide [Met(O)] residues in proteins to methionine. An msrA disruption mutant of M. genitalium, constructed through homologous recombination, displayed markedly reduced adherence to sheep erythrocytes. In addition, the msrA mutant was incapable of growing in hamsters and exhibited hypersensitivity to hydrogen peroxide when compared to wild-type virulent M. genitalium. These results indicate that MsrA plays an important role in M. genitalium pathogenicity, possibly by protecting mycoplasma protein structures from oxidative damage or through alternate virulence-related pathways.