Organ culture of osteopetrotic (ia) rat bone: evidence that the defect is cellular.
Organ culture of osteopetrotic (ia) rat bone: evidence that the defect is cellular.
复制标题
骨石症 (ia) 大鼠骨的器官培养：证据表明缺陷是细胞性的。
作者：
L. Nyberg;S. Marks,
将来自骨硬化（ia）大鼠和正常同窝出生大鼠的颅骨在添加有同源血清的化学成分确定的培养基中培养，以测试突变血清中骨吸收抑制剂的存在或促进剂的不存在。此外，突变体和正常骨的甲状旁腺提取物和氢化可的松的反应进行了测试在体外。结果表明，突变体和正常血清没有不同方面的能力，以支持骨吸收和ia骨响应氢化可的松，但不甲状旁腺提取物在器官培养。这些数据表明ia大鼠的骨骼缺陷不是体液性的而是细胞性的。
Calvarial bone from osteopetrotic (ia) rats and normal littermates has been cultured in a chemically defined medium supplemented with homologous serum to test for the presence of inhibitors or the absence of promoters of bone resorption in mutant serum. In addition, the response of mutant and normal bone to parathyroid extract and hydrocortisone was tested in vitro. The results indicate that mutant and normal serum do not differ with respect to their ability to support bone resorption and that ia bone responds to hydrocortisone but not parathyroid extract in organ culture. These data indicate that the skeletal defect in ia rats is not humoral but cellular.