A single-gene biomarker identifies breast cancers associated with immature cell type and short duration of prior breastfeeding
A single-gene biomarker identifies breast cancers associated with immature cell type and short duration of prior breastfeeding
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DOI：
10.1677/erc.1.01051
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发表时间：
2005-12-01
影响因子：
3.9
通讯作者：
Pusztai, L
中科院分区：
文献类型：
--
作者：
Symmans, WF;Fiterman, DJ;Pusztai, L
雌激素受体或人表皮生长因子受体2（Her - 2/neu）不表达（ER - /HER2 - 表型）的乳腺癌发病机制尚未完全明确。我们观察到在一些具有ER - /HER2 - 表型的乳腺癌中，γ - 氨基丁酸A 型（GABA(A)）受体π亚单位（GABA π，GABRP）基因表达显著升高。在本研究中，通过寡核苷酸微阵列获取了82例原发性浸润性乳腺癌的转录图谱（TxPs）。实时逆转录 - 聚合酶链反应（RT - PCR）用于检测另一组121例浸润性乳腺癌中GABA π基因的表达。对来自转录图谱和实时逆转录 - 聚合酶链反应的GABA π基因表达值进行标准化，并与203例患者的临床病理特征进行比较。16%的乳腺癌中GABA π基因表达增加（转录图谱检测为113/82，实时逆转录 - 聚合酶链反应检测为20/121），特别是在具有ER - /HER2 - 表型（60%）以及具有基底样基因组图谱（60%）的乳腺癌中。在这些肿瘤中与GABA π共同表达的基因图谱与未成熟细胞类型一致。在多变量线性回归分析中，GABA π基因表达水平与ER - /HER2 - 表型相关（P<...此处似乎原文未完整给出P值相关内容）
The pathogenesis of breast cancers that do not express estrogen receptors or Her-2/neu receptors (ER-/HER2- phenotype) is incompletely understood. We had observed markedly elevated gene expression of gamma-aminobutyric acid type A (GABA(A)) receptor subunit pi (GABA pi, GABRP) in some breast cancers with ER-/HER2- phenotype. In this study, transcriptional profiles (TxPs) were obtained from 82 primary invasive breast cancers by oligonucleotide microarrays. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to measure GABA pi gene expression in a separate cohort of 121 invasive breast cancers. GABA pi: gene expression values from TxP and RT-PCR were standardized and compared with clinicopathologic characteristics in the 203 patients. GABA pi gene expression was increased in 16% of breast cancers (113/82 TxP, 20/ 121 RT-PCR), particularly in breast cancers with ER-/HER2- phenotype (60%), and breast cancers with basal-like genomic profile (60%). The profile of genes coexpressed with GABA pi in these tumors was consistent with an immature cell type. In multivariate linear regression analysis, the level of GABA pi gene expression was associated with ER-/HER2- phenotype (P