NEW TYPE OF POU DOMAIN IN GERM LINE-SPECIFIC PROTEIN OCT-4
NEW TYPE OF POU DOMAIN IN GERM LINE-SPECIFIC PROTEIN OCT-4
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DOI：
10.1038/344435a0
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发表时间：
1990-03-29
期刊：
影响因子：
64.8
通讯作者：
GRUSS, P
中科院分区：
文献类型：
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作者：
SCHOLER, HR;RUPPERT, S;GRUSS, P
鼠八聚体结合蛋白家族的成员与八聚体基序特异性相互作用，八聚体基序是在许多基因的启动子和增强子区域中发现的转录调控元件1。Oct-4是一种母体表达的蛋白质，也存在于植入前小鼠胚胎中1，2。尽管许多调节蛋白在着床后胚胎中表达3，但调节着床前过程的转录因子仍然难以捉摸。因此，Oct-4基因是早期发育控制基因的主要候选者。本文报道了小鼠Oct-4基因的互补DNA克隆，并通过体外转录、翻译、DNA结合和蛋白酶剪切分析，鉴定了其编码蛋白。缺失分析表明，DNA结合活性是由一个POU结构域介导的，该结构域编码在一个开放的阅读框架中，对应于一个324个氨基酸的蛋白质4 -13。与已知POU结构域的序列比较表明Oct-4是POU家族的一个新成员。
MEMBERS of a family of murine octamer-binding proteins inter-act specifically with the octamer motif, a transcription regulatory element found in the promoter and enhancer regions of many genes1. Oct-4 is a maternally expressed protein that is also present in the pre-implantation mouse embryo1,2. Although many regula-tory proteins are expressed in post-implantation embryos3, tran-scription factors regulating pre-implantation processes have remained elusive. TheOct-4gene is therefore a prime candidate for an early developmental control gene. Here we report the complementary DNA cloning of the mouseOct-4gene, and the characterization of the encoded protein(s) by sequentialin vitrotranscription, translation, DNA-binding and protease-clipping analysis. Deletion analysis shows that the DNA-binding activity is mediated by a POU domain encoded in an open reading frame corresponding to a 324-amino-acid protein4–13. Sequence com-parison with known POU domains reveals that Oct-4 is a novel member of the POU-family.