Evaluation of methods for the measurement of low-density lipoprotein cholesterol
Evaluation of methods for the measurement of low-density lipoprotein cholesterol
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DOI：
10.1177/107424840501000106
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发表时间：
2005-03-01
影响因子：
2.6
通讯作者：
Elisaf, MS
中科院分区：
文献类型：
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作者：
Bairaktari, ET;Seferiadis, KI;Elisaf, MS
血浆中高浓度的低密度脂蛋白胆固醇（LDL-C）是动脉粥样硬化性心血管疾病和死亡的最强危险因素之一。临床实验室测定 LDL-C 最常用的方法是 Friedewald 计算。人们越来越关注提高通过该方程估计的 LDL-C 的准确性。国家胆固醇教育计划召集的专家小组建议开发准确的直接方法来测量 LDL-C。近年来，人们引入了几种同质且全自动的方法，这些方法显示出比早期方法（尤其是弗里德瓦尔德计算）更高的精度和准确度。血浆中的每个致动脉粥样硬化颗粒（极低、中密度和低密度脂蛋白）以及脂蛋白（a）均含有一个载脂蛋白B（apoB）分子，因此，血浆中apoB的总浓度反映了致动脉粥样硬化颗粒的数量。多项研究表明，apoB 的测量可以提高冠状动脉疾病风险的预测。因此，除了新开发的直接测定法之外，还提出了替代计算程序，该程序还考虑了用于估计 LDL-C 的总血清 apoB 浓度以及小而密的 LDL 颗粒的存在。新一代LDL-C同质测量方法以及利用血清apoB浓度估算LDL-C有助于准确测定LDL-C。
A high concentration of low-density lipoprotein cholesterol (LDL-C) in plasma is one of the strongest risk factors for atherosclerotic cardiovascular disease and mortality. The most common approach to determining LDL-C in the clinical laboratory is the Friedewald calculation. There is an increased interest to improve the accuracy of LDL-C estimated by this equation. The expert panel convened by National Cholesterol Education Program has recommended the development of accurate direct methods to measure LDL-C. Several homogeneous and fully automated methods have been introduced in recent years that show improved precision and accuracy over earlier methods, especially the Friedewald calculation. Each of the atherogenic particles in plasma-very-low, intermediate-, and low- density lipoprotein-as well as lipoprotein (a), contain one molecule of apolipoprotein B (apoB) and thus, plasma total concentration of apoB reflects the number of atherogenic particles. Several studies suggested that the measurement of apoB could improve the prediction of risk of coronary artery disease. Thus, in addition to the newly developed direct assays, alternative calculation procedures have been proposed that also take into consideration total serum apoB concentration for the estimation of LDL-C and the presence of small, dense LDL particles. The new generation of homogenous methods for the measurement of LDL-C and the use of serum apoB concentration for the estimation of LDL-C can contribute to the accurate LDL-C determination.