Effect ofBacillus velezensis JC-K3 on Endophytic Bacterial and Fungal Diversity in Wheat Under Salt Stress.
Effect ofBacillus velezensis JC-K3 on Endophytic Bacterial and Fungal Diversity in Wheat Under Salt Stress.
复制标题
绒毛芽孢杆菌JC-K3对盐胁迫下小麦内生细菌和真菌多样性的影响
DOI：
10.3389/fmicb.2021.802054
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发表时间：
2021
影响因子：
5.2
通讯作者：
Cao H
中科院分区：
文献类型：
--
作者：
Ji C;Wang X;Song X;Zhou Q;Li C;Chen Z;Gao Q;Li H;Li J;Zhang P;Cao H
植物促生菌（PGPB）能有效减轻植物盐害。目前，关于PGPB对盐胁迫下根际土壤微生物群落结构的影响研究较多，而对植物内生细菌和真菌群落结构的研究较少。我们认为，内生细菌接种到植物根际可以显着影响植物的地上和地下部分的微生物群落结构，这可能是植物的“诱导系统耐受性”的原因。将分离的内生菌重新接种到盐胁迫下的根际。结果表明，与对照组相比，接种内生芽孢杆菌JC-K3不仅增加了小麦生物量的积累，而且提高了小麦体内可溶性糖和叶绿素的含量，降低了小麦地上部和叶片对Na的吸收。接种JC-K3后，地上部和叶片中细菌群落的丰度增加，真菌群落的丰度降低。小麦根际土壤真菌群落丰富度显著增加。地上部和叶片中细菌群落多样性增加，真菌群落丰富度下降。JC-K3菌株提高了小麦的生物量积累能力、渗透调节能力和离子选择性吸收能力。此外，JC-K3显著改变了小麦内生和根际微生物的多样性和丰度。PGPB能有效减轻植物盐害。目前，关于盐胁迫下PGPB对根际土壤微生物群落结构影响的研究较多，但对植物内生细菌和真菌群落结构变化的研究较少。
Plant growth-promoting bacteria (PGPB) can effectively reduce salt damage in plants. Currently, there are many studies on the effects of PGPB on the microbial community structure of rhizosphere soil under salt stress, but fewer studies on the community structure of endophytic bacteria and fungi. We propose that inoculation of endophytic bacteria into the rhizosphere of plants can significantly affect the microbial community structure of the plant’s above-ground and underground parts, which may be the cause of the plant’s “Induced Systemic Tolerance.” The isolated endophytes were re-inoculated into the rhizosphere under salinity stress. We found that, compared with the control group, inoculation with endophytic Bacillus velezensis JC-K3 not only increased the accumulation of wheat biomass, but also increased the content of soluble sugar and chlorophyll in wheat, and reduced the absorption of Na in wheat shoots and leaves. The abundance of bacterial communities in shoots and leaves increased and the abundance of fungal communities decreased after inoculation with JC-K3. The fungal community richness of wheat rhizosphere soil was significantly increased. The diversity of bacterial communities in shoots and leaves increased, and the richness of fungal communities decreased. JC-K3 strain improved wheat’s biomass accumulation ability, osmotic adjustment ability, and ion selective absorption ability. In addition, JC-K3 significantly altered the diversity and abundance of endophytic and rhizosphere microorganisms in wheat. PGPB can effectively reduce plant salt damage. At present, there are many studies on the effect of PGPB on the microbial community structure in rhizosphere soil under salt stress, but there are few studies on the community structure changes of endophytic bacteria and fungi in plants.