Elucidation of the β-carotene hydroxylation pathway in Arabidopsis thaliana
Elucidation of the β-carotene hydroxylation pathway in Arabidopsis thaliana
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DOI：
10.1016/j.febslet.2006.07.055
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发表时间：
2006-08-21
期刊：
影响因子：
3.5
通讯作者：
Giuliano, Giovanni
中科院分区：
文献类型：
--
作者：
Fiore, Alessia;Dall'Osto, Luca;Giuliano, Giovanni
植物叶黄素生物合成的第一步是类胡萝卜素羟化。在拟南芥中，这一反应是由血红素(LUT1和LUT5)和非血红素(CHY1和CHY2)羟基酶执行的。到目前为止，还没有完全取消α-或β-胡萝卜素羟化的突变体被描述。我们构建了CHY1、CHY2、LuTi、LUT5和LUT2(番茄红素表观环化酶)的双突变和三突变组合。在chy1chy2lut2中，80%的叶片类胡萝卜素由β-胡萝卜素代表。在chy1chy2lut5中，β-胡萝卜素的羟化完全被取消，而α-胡萝卜素的β-环羟化仍可观察到。这些数据与LUT5在β-环羟化中的作用以及作用于α-胡萝卜素而不是β-胡萝卜素的β-环上的额外羟基酶的存在是一致的。(C)2006年，由Elsevier B.V.代表欧洲生化学会联合会出版。
The first dedicated step in plant xanthophyll biosynthesis is carotenoid hydroxylation. In Arabidopsis thaliana, this reaction is performed by both heme (LUT1 and LUT5) and non-heme (CHY1 and CHY2) hydroxylases. No mutant completely abolishing alpha- or beta-carotene hydroxylation has been described to date. We constructed double and triple mutant combinations in CHY1, CHY2, LUTI, LUT5 and LUT2 (lycopene epsiloncyclase). In chy1chy2lut2, 80% of leaf carotenoids is represented by beta-carotene. In chy1chy2lut5, beta-carotene hydroxylation is completely abolished, while hydroxylation of the beta-ring of alpha-carotene is still observed. The data are consistent with a role of LUT5 in beta-ring hydroxylation, and with the existence of an additional hydroxylase, acting on the beta-ring of alpha-, but not beta-carotene. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.