A SURFACE PROTEIN EXPRESSED BY AVIAN MYELINATING AND NONMYELINATING SCHWANN-CELLS BUT NOT BY SATELLITE OR ENTERIC GLIAL-CELLS
A SURFACE PROTEIN EXPRESSED BY AVIAN MYELINATING AND NONMYELINATING SCHWANN-CELLS BUT NOT BY SATELLITE OR ENTERIC GLIAL-CELLS
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DOI：
10.1016/0896-6273(88)90141-9
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发表时间：
1988-05-01
期刊：
影响因子：
16.2
通讯作者：
LEDOUARIN, NM
中科院分区：
文献类型：
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作者：
DULAC, C;CAMERONCURRY, P;LEDOUARIN, NM
为了寻找神经嵴衍生细胞谱系的特异性标记物，我们用从成年鹌鹑外周髓磷脂中纯化的糖蛋白免疫小鼠。我们获得了与髓鞘和外周神经胶质细胞反应的单克隆抗体。这种针对雪旺氏细胞髓鞘蛋白（SMP）的抗体对所有雪旺氏细胞的膜都是特异性的，无论它们是否与有髓鞘神经相关。SMP在体外长期培养的雪旺细胞上持续存在，但在体内和体外均不存在于外周神经节的卫星细胞中。抗原（M的蛋白质双联体，75，000 - 80，000）存在于但不限于髓磷脂层中，因为它沿着整个髓鞘形成的许旺细胞膜分布。在CNS中，SMP表现为M的单一条带，80，000。SMP首先在鹌鹑的E6通过免疫荧光检测到，这比已经描述的与髓鞘形成相关的标志物的首次出现早至少6天。
Searching for specific markers of neural crest-derived cell lineages, we immunized mice with glycoproteins purified from adult quail peripheral myelin. we obtained a monoclonal antibody that reacts with myelin and peripheral glial cells. This antibody, to Schwann cell myelin protein(SMP), is specific for the membranes of all Schwann cells, irrespective of whether they are associated with myelinated nerves. SMP persists on Schwann cells in long-term cultures in vitro, but is absent from satellite cells of peripheral ganglia, both in vivo and in vitro. The antigen(a protein doublet of M, 75,000-80,000) is present in, but not restricted to, the myelin lamellae, since it is distributed along the whole myelinating Schwann cell membrane. In the CNS, SMP appears as a single band of M, 80,000. SMP is first detectable by immunofluorescence at E6 in the quail, which is at least 6 days earlier than the first appearance of already described markers related to myelination.