A novel device for real-time measurement and manipulation of licking behavior in head-fixed mice.
A novel device for real-time measurement and manipulation of licking behavior in head-fixed mice.
复制标题
一种用于实时测量和操纵固定头部小鼠舔舐行为的新型装置。
DOI：
10.1152/jn.00500.2018
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发表时间：
2018
影响因子：
2.5
通讯作者：
Haider,Bilal
中科院分区：
文献类型：
--
作者：
Williams,Brice;Speed,Anderson;Haider,Bilal
小鼠已成为研究哺乳动物神经系统的重要模型系统。在老鼠对感官刺激做出反应的过程中，通过舔舐液体奖励，技术可以记录和操纵神经回路。在这些任务中对舔舐的精确监测提供了一种可访问的感觉-运动处理度量，特别是当与同时的神经记录相结合时。在小鼠固定头部神经生理实验中，舔舐检测器的设计和实现存在一些挑战。首先，老鼠很小，舔舐行为很容易受到大型传感器的干扰或影响。其次，舔舐过程中的神经记录对电接触伪影高度敏感。第三，需要亚毫秒级的舔舐检测延迟来生成控制信号，以在适当的时间尺度上操纵神经活动。在这里，我们设计、表征并实现了一种非接触式双端口设备，该设备可以精确测量头部固定小鼠执行视觉行为的定向舔舐。我们首先通过设计迭代确定了探测器的最佳特性，然后量化了理想条件下的器件性能。然后，我们在体内用同步神经记录测试了头部固定小鼠行为的表现。我们最终证明了我们的设备能够检测定向舔舐，并实时产生适当的控制信号，通过闭环抑制神经活动来快速抑制舔舐行为。我们的双端口检测器具有成本效益和易于复制，它应该能够在正常和转基因小鼠模型中广泛应用于探测感觉知觉，运动动作和学习的神经回路基础。新的和值得注意的ymice很容易学习任务，他们通过舔舐液体奖励来响应感官线索；涉及多次舔舐反应的任务允许研究决策和感觉-运动整合的神经回路。在这里，我们设计、表征并实现了一种新的双端口舔舐检测器，它可以精确测量头部固定小鼠进行视觉行为的定向舔舐，从而实现同时的神经记录和舔舐闭环操作。
The mouse has become an influential model system for investigating the mammalian nervous system. Technologies in mice enable recording and manipulation of neural circuits during tasks where they respond to sensory stimuli by licking for liquid rewards. Precise monitoring of licking during these tasks provides an accessible metric of sensory-motor processing, particularly when combined with simultaneous neural recordings. There are several challenges in designing and implementing lick detectors during head-fixed neurophysiological experiments in mice. First, mice are small, and licking behaviors are easily perturbed or biased by large sensors. Second, neural recordings during licking are highly sensitive to electrical contact artifacts. Third, submillisecond lick detection latencies are required to generate control signals that manipulate neural activity at appropriate time scales. Here we designed, characterized, and implemented a contactless dual-port device that precisely measures directional licking in head-fixed mice performing visual behavior. We first determined the optimal characteristics of our detector through design iteration and then quantified device performance under ideal conditions. We then tested performance during head-fixed mouse behavior with simultaneous neural recordings in vivo. We finally demonstrate our device’s ability to detect directional licks and generate appropriate control signals in real time to rapidly suppress licking behavior via closed-loop inhibition of neural activity. Our dual-port detector is cost effective and easily replicable, and it should enable a wide variety of applications probing the neural circuit basis of sensory perception, motor action, and learning in normal and transgenic mouse models.NEW & NOTEWORTHYMice readily learn tasks in which they respond to sensory cues by licking for liquid rewards; tasks that involve multiple licking responses allow study of neural circuits underlying decision making and sensory-motor integration. Here we design, characterize, and implement a novel dual-port lick detector that precisely measures directional licking in head-fixed mice performing visual behavior, enabling simultaneous neural recording and closed-loop manipulation of licking.